Overview

Transcription is part of the process of gene expression and is very different in prokaryotes and eukaryotes. In prokaryotic cells the synthesis reactions of DNA, RNA and proteins are intricately coupled as there are no subcellular compartments. Thus replication, transcription and translation occur simultaneously without local separation and the regulations of each is inter-related. In bacteria, regulation is most important at the transcription stage of the biosynthetic process, and changes in the transcriptional efficiency account for major differences in the frequency of RNA formation and gene expression. This textbook has been written by an authority in the field for advanced students and postgraduates. Both clear and concise, there is an emphasis on transcription in bacterial and phage expression systems, a key area of study and research in molecular biology. Many general mechanisms and principles emerge from studies of prokaryotic transcription complexes that help build an understanding of regulation in higher organisms. Taking a biophysical approach, the text draws together elements of molecular biology, genetics, structural biology and biochemistry to provide an invaluable summary of the field of prokaryotic transcription regulation for students in molecular biology and related areas of study such as microbiology and biotechnology. Additional content information: this book includes -new data on the composition and architecture of RNA polmerase - new findings on sigma- and anti-sigma- factors -a description of promoter elements underlining the importance of upstream and downstream flanking sequences -detailed kinetic description of the initiation process -new data on the function of DNA curvature and topology on transcription -general mechanisms of repression -positive regulation -termination, anti-termination and attenuation mechanisms -implications of the nascent RNA structure in regulation -stringent control mechanisms and global networks -comparative chapter for parallel and divergent mechanisms in prokaryotic and eukaryotic transcription regulation -modern methods of analysis and new technical developments with selected examples

Full Product Details

Author:   Rolf Wagner (Institute for Physical Biology, Institute for Physical Biology, Heinrich-Heine University, Dusseldorf, Germany)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Dimensions:   Width: 15.60cm , Height: 2.00cm , Length: 23.40cm
Weight:   0.593kg
ISBN:  

9780198503545

ISBN 10:   0198503547
Pages:   380
Publication Date:   18 May 2000
Audience:   College/higher education ,  Tertiary & Higher Education
Format:   Paperback
Publisher's Status:   Active
Availability:   To order  
Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us.

Table of Contents

1 General introduction Flow of genetic information Distinctive features of prokaryotic gene regulation Biochemical nature of the transcription reaction What makes RNA transcription different from DNA replication? The products of transcription 2 The players or cellular components necessary for transcription 2.1 Transcription Start sites: the promoter 2.2 RNA polymerase 2.3 Alternative ( factors 2.4 The 3-dimensional structure of RNA polymerase 2.5 Is RNA polymerase modified during different stages of transcription? Initiation of transcription 3.1 Promotor location 3.2 The closed RNA polymerase-promotor complex 3.3 Formation of open complexes 3.4 Binding of substrate NTPs the ternary complexes Promotor clearance and the formation of elongating complexes The thermodynamics and kinetics of transcription initiation Transcription elongation 4.1 The architecture of elongating complexes what is known about the different functional site? 4.2 The rate of elongation 4.3 RNA polymerases during transcription 4.4 Transcript cleavage factors 'Traffic problems' at the bacterial genome inevitable collisions between transcription and replication machineries Termination of transcription 5.1 Factor-independent termination 5.2 Factor dependent termination 5.3 Attenuation of transcription 5.4 Antitermination The role of DNA structure in transcription regulation 6.1 Is DNA methylation involved in regulation? 6.2 Effects of DNA curvature on transcription inhibition 6.3 DNA supercoiling and transcription Regulation by transcription factors 7.1 Repressors and operators the classical model 7.2 The modular structure of transcription factors 7.3 LysR a representative of a large transcription factor family 7.4 DNA loops regulation through dynamic structures regulation by DNA structuring proteins Two component regulatory systems Regulatory networks 8.1 The SOS response 8.2 The heat-shock response 8.3 Stationary phase control 8.4 The Lrp regulon The stringent control Growth rate regulation Lessons from a many-fold regulated system the synthesis of ribosomal RNA

Reviews

I therefore regard this book as a standard, extremely suitable not only for teaching to 3rd or 4th year undergraduate students with interest in cellular biology and molecular microbiology, but also for senior scientists who have research interests in prokaryotic transcription regulation2 Cell Biology International 'a superb, compact yet comprehensive, treatise on the regulation of gene expression, principally but not exclusively, in E.Coli and its phage ... A must for all students at undergraduate or postgraduate level and also for reseachers of eukaryotic transcription who need reminding of a few paradigms' Aslib

"""I therefore regard this book as a standard, extremely suitable not only for teaching to 3rd or 4th year undergraduate students with interest in cellular biology and molecular microbiology, but also for senior scientists who have research interests in prokaryotic transcription regulation2 Cell Biology International 'a superb, compact yet comprehensive, treatise on the regulation of gene expression, principally but not exclusively, in E.Coli and its phage ... A must for all students at undergraduate or postgraduate level and also for reseachers of eukaryotic transcription who need reminding of a few paradigms' Aslib"

'a superb, compact yet comprehensive, treatise on the regulation of gene expression, principally but not exclusively, in E.Coli and its phage ... A must for all students at undergraduate or postgraduate level and also for reseachers of eukaryotic transcription who need reminding of a few paradigms' Aslib I therefore regard this book as a standard, extremely suitable not only for teaching to 3rd or 4th year undergraduate students with interest in cellular biology and molecular microbiology, but also for senior scientists who have research interests in prokaryotic transcription regulation2 Cell Biology International

Author Information

Prof Rolf Wagner runs the Institute of Physical Biology and teaches part of the molecular biology undergraduate course at the University of Dusseldorf. He has been involved in active research and teaching of prokaryotic transcription control for over ten years.

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