Overview

This book collects protocols from different areas of knowledge to assist in the identification of toxic effects exerted by different xenobiotics. At the same time as classical techniques are presented, modern techniques with alternative models to the use of animals are also presented. Given the ever-increasing exposure to different compounds and their effects on population health, the assessment of multiple endpoints is of utmost importance for better risk assessment, and this collection addresses that need. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Toxicity Assessment: Methods and Protocols aims to serve researchers in this vast field of science as they seek to better understand the mechanisms of action of different xenobiotics.

Full Product Details

Author:   Carlos Manuel Marques Palmeira ,  Danielle Palma de Oliveira ,  Daniel Junqueira Dorta
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2240
Weight:   0.537kg
ISBN:  

9781071610930

ISBN 10:   1071610937
Pages:   266
Publication Date:   10 January 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. The Single Cell Gel Electrophoresis Genotoxin Sensitivity Assay             Franziska Ferk, Miroslav Mišík, Halh Al-Serori, Armen Nersesyan, Georg Wultsch, and Siegfried Knasmueller   2. Skin Sensitization Tests: The LLNA and the RhE IL-18 Potency Assay             Emanuela Corsini, Sue Gibbs, Erwin Roggen, Ian Kimber, and David A. Basketter   3. Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation             Tatiana do Nascimento Pedrosa, Carolina Motter Catarino, Paula Comune Pennacchi, Silvia Berlanga de Moraes Barros, and Silvya Stuchi Maria-Engler   4. Intravital Microscopy: A Tool to Investigate the Toxicity in the Immune System, Vessel Rheology, and Xenobiotic Distribution             Cristina Bichels Hebeda, Éric Diego Barioni, and Sandra Helena Poliselli Farsky   5. Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment             Moritoshi Sato and Yoshio Umezawa   6. In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function             Cibele S. Borges, Luiz Ricardo A. Kiguti, André S. Pupo, and Wilma G. Kempinas   7. Early Life Stage Assays in Zebrafish             Flavia Renata Abe, Klaus Alvaro Guerrieri Accoroni, Carlos Gravato, and Danielle Palma de Oliveira   8. Assessment of Urothelial Cytotoxicity at Morphological and Molecular Levels: Urinary Bladder as Target Organ             Thania Rios Rossi Lima, Bianca Camargo Penteado Sales, and Shadia Muhammad Ihlaseh Catalano   9. Evaluation of Development of the Rat Uterus as a Toxicity Biomarker             Marlise Guerrero Schimpf, María M. Milesi, Enrique H. Luque, and Jorgelina Varayoud   10. Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish             Carlos Gravato, Flávia R. Abe, Danielle P. Oliveira, Amadeu M.V.M. Soares, and Inês Domingues   11. Ecotoxicity Assays Using Freshwater Planarians             João Luís Teixeira Pestana and Pearl Ugochi Ofoegbu   12. Toxicogenomic Analysis             Taisa Magnani Dinamarco, Paula Fagundes de Gouvêa, Emerson de Souza Santos, and Lucas Oliveira Sousa   13. Strategy Combining Non-Animal Methods for Ocular Toxicity Evaluation             Marize Campos Valadares, Gisele Augusto Rodrigues de Oliveira, Renato Ivan de Ávila, and Artur Christian Garcia da Silva   14. Immunocytochemistry Analysis of HepG2 Cell 3D Culture Encapsulated as Spheroids in Alginate Beads             Raul Ghiraldelli Miranda, Elisa Raquel Anastácio Ferraz, Lilian Cristina Pereira, and Daniel Junqueira Dorta   15. Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function and Cell Fate Along Neural Deregulation             Mariana Dutra Brito, Luiz Felipe Souza e Silva, Amanda Siena, Miruna Chipara, Sovan Sarkar, and Tatiana Rosenstock   16. Mitochondrial Bioenergetic Assays as a Standard Protocol for Toxicological and Metabolic Assessment             João Soeiro Teodoro, Anabela Pinto Rolo, and Carlos Manuel Marques Palmeira   17. In Vitro Apoptotic Cell Death Assessment             Lílian Cristina Pereira, Alecsandra Oliveira de Souza, and Daniel Junqueira Dorta

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