Overview

This introduction to the scientific basis of histochemistry provides a background for the selection and development of appropriate methods. The topic is treated by striking a balance between the traditional empirical techniques and the modern highly specialized and sophisticated methods. The book underscores the fact that all histological and histopathological methods are essentially histochemical in nature. Recognition of this leads to the realization that a clear understanding of the principles set out in the book are the basis for the acceptance of comparable, comprehensible and standardizable staining protocols, even for the classical empirically derived techniques. The book is organized into seven sections: general considerations, a review of techniques according to mechanism, tissue processing, the staining of chemical entities, enzyme histochemistry, other techniques and an introduction to applied histochemistry. The text has been completed with a ""Note to the Reader"" so that readers with different backgrounds can select the sections and sequences most appropriate for their own particular requirements. This monograph on histochemistry, pathology and anatomy is intended for researchers, students and technicians.

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9783540193111

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