Overview

This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.

Full Product Details

Author:   H. William Detrich III (Professor of Biochemistry and Marine Biology at Northeastern University) ,  William H. Detrich (Northeastern University, Boston, MA, USA) ,  William H. Detrich (Northeastern University, Boston, MA, USA) ,  William H. Detrich (Northeastern University, Boston, MA, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   3rd edition
Volume:   135
Weight:   1.300kg
ISBN:  

9780123748140

ISBN 10:   0123748143
Pages:   528
Publication Date:   24 October 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Replaced By:   9780128034743
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Part I: Forward and Reverse Genetics 1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish – Wenbiao Chen 2. Advances in Tol2 Transposon Mutagenesis – Koichi Kawakami 3. OPEN Zinc Finger Nucleases – Keith Joung and Randy Peterson 4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish – Adam Amsterdam, Nancy Hopkins, and Shawn Burgess 5. Genetic Screens for Maternal-Effects Mutations – Francisco Pelegri and Mary Mullins 6. High-Throughput Target-Selected Gene Inactivation in Zebrafish – Derek Stemple 7. Suppressor Screens in Haploids – Xiaoying Bai 8. Transposons – Stephen Ekker 9. Caged MOs – James Chen Part II: Transgenesis 1. Advanced Transgenesis with Cre Recombinase – Christian Mosimann 2. Transgenesis with phiC31 Recombinase – James Lister 3. Nuclear Transfer – Jose Cibelli and Dr. Siripattarapravat Part III: The Zebrafish Genome and Mapping Technologies 1. The Zebrafish Genome Project: Sequence Analysis and Annotation– Derek Stemple 2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish – Charles Lee and Amanda Smith 3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome – John Postlethwait, Julian Catchen 4. The Zon Laboratory Guide to Positional Cloning in Zebrafish –Yi Zhou and Leonard Zon Part IV: Informatics and Comparative Genomics 1. Data Warehouse ZFIN – Monte Westerfield and Judy Sprague 2. Epigenetics: Methylation – Brad Cairns 3. Chromatin Structure – Leonard Zon and Yi Zhou 4. ChipSeq – Brad Cairns 5. Micro RNAs – Graham Lieshke and Luke Pase 6. Microarrays – Yi Zhou 7. Sequence-Based Transcriptional Profiling – Shawn Burgess 8. Epigenetic Regulation of Zebrafish Development – Elke Ober and co-author Part V: Infrastructure 1. Zebrafish Facilities for Small and Large Laboratories – Christian Lawrence 2. Genetic Backgrounds, Standard Lines, and their Husbandry – ZIRC, Zoltan

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"Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons ""on the ice"" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms."

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