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Author:   Nicholas Hastie
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2016
Volume:   1467
Dimensions:   Width: 17.80cm , Height: 1.60cm , Length: 25.40cm
Weight:   6.148kg
ISBN:  

9781493940219

ISBN 10:   149394021
Pages:   235
Publication Date:   20 July 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

WT1 Mutation in Childhood Cancer.- WT1 and Renal Disease.- The Role of WT1 in Embryonic Development and Normal Organ Homeostasis.- Tools and Techniques for WT1-Based Lineage Tracing.- Biological Systems and Methods for Studying WT1 in the Epicardium.- Isolation and Colony Formation of Murine Bone and Bone Marrow Cells.- Isolation and Fluorescent Activated Cell Sorting of Murine WT1-Expressing Adipocyte Precursor  Cells.- In Vivo Assays for Assessing the Role of the Wilms’ Tumor Suppressor 1 (WT1) in Angiogenesis.- Multiphoton Microscopy for Visualizing Lipids in Tissue.- Function and Regulation of the Wilm’s Tumor Suppressor 1 (WT1) Gene in Fish.- Immunofluorescence Staining of WT1 on Sections of Zebrafish Embryos and Larvae.- Fluorescence Activated Cell Sorting (FACS) Protocol for Podocyte Isolation in Adult Zebrafish.- In Vitro Transcription to Study WT1 Function.- Measuring Equilibrium Binding Constants for the WT1-DNAInteraction Using a Filter Binding Assay.- Identifying Direct Downstream Targets: WT1 ChIP-Seq Analysis.- WT1 Associated Protein-Protein Interaction Networks.- Methods to Identify and Validate WT1-RNA Interaction.- Bioinformatic Analysis of Next Generation Sequencing Data to Identify WT1 Associated Differential Gene and Isoform Expression.- Immunotherapy Targeting WT1: Designing a Protocol for WT1 Peptide-Based Cancer Vaccine.

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