Overview

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

Full Product Details

Author:   R. A Crowther (MRC Laboratory of Molecular Biology, Cambridge, UK)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   579
Dimensions:   Width: 15.20cm , Height: 2.80cm , Length: 22.90cm
Weight:   0.910kg
ISBN:  

9780128053829

ISBN 10:   0128053828
Pages:   486
Publication Date:   26 August 2016
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Direct Electron Detectors  - Methods in Enzymology (2016) Greg McMullan, Abdul R. Faruqi and Richard Henderson 2. Specimen Behavior in the Electron Beam Robert M. Glaeser 3. Specimen Preparation for High-Resolution Cryo-EM Christopher J. Russo and Lori A. Passmore 4. Strategies for Automated Cryoem Data Collection Using Direct Detectors Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher 5. Processing of Cryo-EM Movie Data Zev A. Ripstein and John L. Rubinstein 6. Processing of Structurally Heterogeneous Cryo-EM Data in RELION Sjors H.W. Scheres 7. Single Particle Refinement and Variability Analysis in EMAN2.1 Steven J. Ludtke 8. Frealign: An Exploratory Tool for Single Particle Cryo-EM Nikolaus Grigorieff 9. Testing the Validity of Single Particle Maps at Low and High Resolution Peter B. Rosenthal 10. Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps Frank DiMaio and Wah Chiu 11. Refinement of Atomic Structures Against CryoEM Maps Garib N. Murshudov 12. Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction Simon A. Fromm and Carsten Sachse 13. Cryo-Electron Tomography and Subtomogram Averaging William Wan and John A.G. Briggs

Reviews

Praise for the Series: Should be on the shelves of all libraries in the world as a whole collection. --Chemistry in Industry The work most often consulted in the lab. --Enzymologia The Methods in Enzymology series represents the gold-standard. --Neuroscience

Praise for the Series: Should be on the shelves of all libraries in the world as a whole collection. --Chemistry in Industry The work most often consulted in the lab. --Enzymologia The Methods in Enzymology series represents the gold-standard. --Neuroscience

Author Information

Tony Crowther is an emeritus scientist at the MRC Laboratory of Molecular Biology in Cambridge, where he was joint head of the Structural Studies Division from 1994 to 2005. He has been involved in electron microscopy and image processing of biological structures for many years. His particular biological interests have been in viruses such as hepatitis B and in the abnormal filaments that form in neurodegenerative diseases. He is a Fellow of the Royal Society of London, a Fellow of the Academy of Medical Sciences and a member of the European Molecular Biology Organization.

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