Overview

This detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.

Full Product Details

Author:   Patrick J. Hussey ,  Pengwei Wang
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2604
Weight:   0.918kg
ISBN:  

9781071628669

ISBN 10:   1071628666
Pages:   369
Publication Date:   12 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy             Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko   2. Actin: Static and Dynamic Studies             Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren   3. 3D Visualization of Microtubules in Epidermal Pavement Cells             Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann   4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells             Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder   5. Single Cell Confinement Methods to Study Plant Cytoskeleton             Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant   6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures             Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot   7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography             Janice Pennington and Marisa S. Otegui   8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)             Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu   9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis             Sabine Müller   10. Expansion Microscopy of Plant Cells (PlantExM)             Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush   11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens             Mari W. Yoshida and Elena Kozgunova   12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens             Yuji Hiwatashi and Takashi Murata   13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools             Fatima Cvrčková and Radek Bezvoda   14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata             Zoe Barr and Jens Tilsner   15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta             Joseph F. McKenna and Katja Graumann   16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination             Lucie Riglet and Isabelle Fobis-Loisy   17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes             Lei Zhu and Ying Fu   18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study             Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó   19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis             Liru Dou, Xiangfeng Wang, and Tonglin Mao   20. Imaging of Cortical Microtubules in Plants under Salt Stress             Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo   21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli             Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li   22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation             Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks   23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes             Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang   24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings             Felix Ruhnow, Staffan Persson, and René Schneider   25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging             Guangda Wang, Yanjun Yu, and Zhaosheng Kong   26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells             Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin   27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato             Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang   28. Light Microscopy Technologies and the Plant Cytoskeleton             Timothy J. Hawkins   29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton             Patrick Duckney and Patrick J. Hussey

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