Overview

This volume covers all methods used to discover the composition, structure, and dynamics of the nuclear pore complex (NPC), as well as the soluble transport factors involved in the transport process. Chapters detail structural biology methods, biophysical, transport factors , and a wide range of model organisms that have been used over the years. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, The Nuclear Pore Complex: Methods and Protocols: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Full Product Details

Author:   Martin W. Goldberg
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2502
Weight:   1.001kg
ISBN:  

9781071623398

ISBN 10:   1071623397
Pages:   515
Publication Date:   13 April 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: The Nuclear Pore Complex and Nucleoporins            1.     Affinity Isolation of Endogenous Saccharomyces cerevisiae Nuclear Pore Complexes Ilona Nudelman, Javier Fernandez-Martinez, and Michael P. Rout   2.     Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes Nikola Kellner and Ed Hurt   3.     Nuclear Pore Complex Assembly using Xenopus Egg Extract Guillaume Holzer and Wolfram Antonin    Part II:  Nucleo-cytoplasmic Passage            4.     Analysis of Nuclear Pore Complex Permeability in Mammalian Cells and Isolated Nuclei Using Fluorescent Dextrans Marcela Raices and Maximiliano A. D’Angelo   5.     Hormone-inducible Transport Reporter Assay to Study Nuclear Import Defects in Neurodegenerative Diseases Saskia Hutten and Dorothee Dormann   6.     Subcellular Fractionation Suitable for Studies of RNA and Protein Trafficking Biljana Culjkovic-Kraljacic and Katherine L.B. Borden   7.     Localising Total mRNA in Plant Cells Geraint Parry   8.     Using Single Molecule RNA FISH to Determine Nuclear Export and Transcription Phenotypes in Drosophila Tissues  Jennifer R. Aleman, Shawn C. Little, and Maya Capelson   Part III: Functional Analysis of Nucleoporins 9.     Analysis of Nucleoporin Function using Inducible Degron Techniques Vasilisa Aksenova, Alexei Arnaoutov, and Mary Dasso   10.  Monitoring of Chromatin Organization at the Nuclear Pore Complex, Inner Nuclear Membrane and Nuclear Interior in Live Cells by Fluorescence Ratiometric Imaging of Chromatin (FRIC) Frida Niss, Cecilia Bergqvist, Anna-Lena Ström, and Einar Hallberg   11.  Analysis of Nuclear Pore Complexes in Caenorhabditis elegans by Live Imaging and Functional Genomic  Patricia de la Cruz Ruiz, Raquel Romero-Bueno, and Peter Askjaer   12.  Protein Retargeting in Aspergillus nidulans to Study the Function of Nuclear Pore Complex Proteins Subbulakshmi Suresh and Stephen A. Osmani   Part IV: Protein-Protein Interactions  13.  Split-GFP Complementation to Study the Nuclear Membrane Proteome using Microscopy. Shary N. Shelton, Sarah E. Smith, and Sue L. Jaspersen 14.  Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin a with Cargo Proteins Alexander Lee, Marie A. Bogoyevitch and David A. Jans   15.  Validation of Nuclear Pore Complex Protein-Protein Interactions by Transient Expression in Plants  Fumika Ikeda and Kentaro Tamura   16.  Binding affinity measurement of Nuclear Export Signal peptides to their exporter CRM1. Ho Yee Joyce Fung, and Yuh Min Chook   Part V: Post Translational Modifications       17.  Analysis of Ubiquitylation and SUMOylation of Yeast Nuclear Pore Complex Proteins Catherine Dargemont   18.  Purification of Cdk-CyclinB-Kinase-Targeted Phosphopeptides from Nuclear Envelope  Justin D. Blethrow, Amanda L. DiGuilio, and Joseph S. Glavy   Part VI: Biophysical Methods 19.  Crystallization of Nuclear Export Signals or Small Molecule Inhibitors Bound to Nuclear Exporter CRM1 Yee Joyce Fung and Yuh Min Chook   20.  Atomic Force Microscopy for Structural and Biophysical Investigations on Nuclear Pore Complexes  Ivan Liashkovich, Gonzalo Rosso, and Victor Shahin   21.  Multivalent Interactions with Intrinsically Disordered Proteins Measured by Surface Plasmon Resonance Larisa E. Kapinos and Roderick Y. H. Lim   22.  Assembly and Use of a Microfluidic Device to Study Nuclear Mechanobiology during Confined Migration Richa Agrawal, Aaron Windsor, and Jan Lammerding   Part VII:  Imaging NPCs and Transport 23.  SPEED Microscopy: High-Speed Single-Molecule Tracking and Mapping of Nucleocytoplasmic Transport Steven J. Schnell, Mark Tingey, and Weidong Yang   24.  Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans Courtney Lancaster, Giulia Zavagno, James Groombridge, Adelaide Raimundo, David Weinkove, Tim Hawkins, Joanne Robson and Martin W. Goldberg   25.  Visualizing Nuclear Pore Complexes in Xenopus Egg Extracts Sampada Mishra and Daniel L. Levy   26.  TEM Imaging of Membrane Choreography During Mitosis of Drosophila Tissue Culture Cells Anton Strunov, Lidiya V. Boldyreva, Alexey V. Pindyurin, Maurizio Gatti, and Elena Kiseleva   27.  Scanning Electron Microscopy (SEM) and Immuno-SEM of Nuclear Pore Complexes from Amphibian Oocytes, Mammalian Cell Cultures, Yeast and Plants Martin W. Goldberg and Jindřiška Fišerová   28.  NPC Structure in Model Organisms: Transmission Electron Microscopy and Immuno-gold Labelling using High Pressure Freezing/Freeze Substitution of Yeast, Worms and Plants  A. Christine Richardson, Jindřiška Fišerová, and Martin W. Goldberg   29.  High Resolution Imaging and Analysis of Individual Nuclear Pore Complexes  Boris Fichtman, Saroj G. Regmi, Mary Dasso, and Amnon Harel   30.  Live CLEM imaging of Tetrahymena to Analyze the Dynamic Behavior of the Nuclear Pore Complex Tokuko Haraguchi, Hiroko Osakada, and Masaaki Iwamoto   31.  Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy Helena Bragulat-Teixidor, M. Julius Hossain, and Shotaro Otsuka

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