Overview

This volume provides an up-to-date collection of protocols describing some of the key methods to investigate the integrated stress response (ISR), a vital evolutionarily conserved mechanism that enables eukaryotic cells to adapt to stress conditions and alter their gene expression programs. The content of the book is split between techniques to analyze mRNA translation regulation and methods to analyze interaction networks and ribonucleoprotein (RNP) granules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, The Integrated Stress Response: Methods and Protocols serves as an ideal guide to help accelerate research into the complex and fascinating biology of the ISR.

Full Product Details

Author:   Daniel Matějů ,  Jeffrey A. Chao
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2428
Weight:   0.787kg
ISBN:  

9781071619773

ISBN 10:   1071619772
Pages:   404
Publication Date:   17 February 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Analysis of mRNA Translation Regulation   1. An Overview of Methods for Detecting eIF2α Phosphorylation and the Integrated Stress Response             Agnieszka Krzyzosiak, Aleksandra Pitera, and Anne Bertolotti   2. CRISPR-Based Screening for Stress Response Factors in Mammalian Cells             Xiaoyan Guo and Martin Kampmann   3. Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq             Gemma E. May and C. Joel McManus   4. Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response             Alyssa M. English and Stephanie L. Moon   5. Quantitative Translation Proteomics Using mePROD             Kevin Klann and Christian Münch   6. Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2             Martin D. Jennings and Graham D. Pavitt   7. Mammalian In Vitro Translation Systems             Yulia Gonskikh, Valentina Pecoraro, and Norbert Polacek   8. Measuring Repeat-Associated Non-AUG (RAN) Translation             Shaopeng Wang and Shuying Sun   9. Analysis of Ribosome Profiling Data             Carine Legrand, Khanh Dao Duc, and Francesca Tuorto   10. Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling             Michael J. Holmes, Jagannath Misra, and Ronald C. Wek   11. High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation             Mohammad Shafieinouri, Britnie Santiago Membreno, and Colin Chih-Chien Wu   12. Fluorescence Intensity-Based eIF2B’s Guanine Nucleotide-Exchange Factor Activity Assay             Yusuke Sekine, David Ron, and Alisa F. Zyryanova   Part II: Analysis of Interaction Networks and RNP Granules   13. Collective Learnings of Studies of Stress Granule Assembly and Composition             Hadjara Sidibé and Christine Vande Velde   14. Detecting Stress Granules in Drosophila Neurons             Fabienne De Graeve, Nadia Formicola, Kavya Vinayan Pushpalatha, Akira Nakamura, Eric Debreuve, Xavier Descombes, and Florence Besse   15. Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation             Anna-Carina Söhnel, Nataliya I. Trushina, and Roland Brandt   16. Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics             Xiaojing Sui, Mona Radwan, Dezerae Cox, and Danny M. Hatters   17. Analyzing the Composition and Organization of Ribonucleoprotein Complexes by APEX-Seq             Alejandro Padrón and Nicholas Ingolia   18. Differential Analysis of the Nuclear and the Cytoplasmic RNA Interactomes in Living Cells             Michael Backlund and Andreas E. Kulozik   19. Identification of RNA-RBP Interactions in Subcellular Compartments by CLIP-Seq             Sonu Sahadevan, Manuela Pérez-Berlanga, and Magdalini Polymenidou   20. Monitoring Virus-Induced Stress Granule Dynamics Using Long-Term Live-Cell Imaging             Vera Magg, Philipp Klein, and Alessia Ruggieri   21. Single-Molecule Imaging of mRNA Interactions with Stress Granules             Tatsuya Morisaki and Timothy J. Stasevich   22. Image-Based Screening for Stress Granule Regulators             Katharina Hoerth, Nina Eiermann, Jürgen Beneke, Holger Erfle, and Georg Stoecklin   23. APEX Proximity Labeling of Stress Granule Proteins             Sara Elmsaouri, Sebastian Markmiller, and Gene W. Yeo

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