Overview

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more. Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L.

Full Product Details

Author:   Clément Thomas (Group Leader, Cytoskeleton and Cancer Progression, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg) ,  Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA)
Publisher:   Elsevier Science & Technology
Imprint:   Academic Press Inc
Weight:   0.590kg
ISBN:  

9780323901543

ISBN 10:   0323901549
Pages:   206
Publication Date:   11 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators Marie Juzans, Céline Cuche, Vincenzo Di Bartolo, and Andrés Alcover 2. Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse Sara Fernández-Hermira, Irene Sanz-Fernández, Marta Botas, Víctor Calvo, and Manuel Izquierdo 3. P815-based redirected degranulation assay to study human NK cell effector functions Iñigo Terrén, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego 4. Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging Yanting Zhu and Jue Shi 5. Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay Ondrej Cerny 6. Assessment of membrane lipid state at the natural killer cell immunological synapse Jordan Orange and Yu Li 7. Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi 8. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto 9. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers Melanie Grusdat, Catherine Dostert, and Dirk Brenner 10. An in vitro model to monitor natural killer cell effector functions against primary breast cancer Nicky A. Beelen, Femke A. I. Ehlers, Loes F. S. Kooreman, Gerard M. J. Bos, and Lotte Wieten 11. Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar Lipid Bilayer Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu 12. Potency Monitoring of Therapeutic Car T Cells Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine Brown

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Author Information

Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

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