Overview

This volume details a comprehensive range of methods for imaging  epithelial-to-mesechymal transition (EMT)/MET in in vivo systems, and methods to leverage these systems to dissect the underlying mechanisms. Chapters guide readers through studying different features of epithelial-mesenchymal plasticity, past and future research of the EMT, in vivo systems, and in vivo imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Epithelial-to Mesenchymal Transition: Methods and Protocols aims to provide methods in EMT will help to unite and drive research in this exciting field forwards.

Full Product Details

Author:   Kyra Campbell ,  Eric Theveneau
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2179
Weight:   0.846kg
ISBN:  

9781071607817

ISBN 10:   1071607812
Pages:   429
Publication Date:   18 September 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

A Spectrum of Cell States during the Epithelial-To-Mesenchymal Transition.- Perspective on Epithelial Mesenchymal Transitions in Embryos.- EMT, One of Many Morphological Transitions in Cellular Phase Space.- Are you Interested or Afraid of Working on EMT?.- Partial EMT/MET: An Army of One.- EMT; An Update.- Mesenchymal to Epithelial Transitions in Development and Cancer.- Live Imaging of Epithelial-mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos.- Zebrafish Neural Crest: Lessons and Tools to Study in vivo Cell Migration.- Live Imaging of the Neural Crest Cell Epithelial-to-Mesenchymal Transition in the Chick Embryo.- Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors.- Visualizing Mouse Embryo Gastrulation Epithelial-mesenchymal Transition through Single Cell Labeling Followed by ex vivo Whole Embryo Live Imaging.- Fluorescence Recovery after Photobleaching to Study the Dynamics of Membrane Bound Proteins in vivo using the Drosophila Embryo.- Methods to Generate and Assay for Distinct Stages of Cancer Metastasis in adult Drosophila melanogaster.- 4D Live-Imaging and Analysis of Chick Embryo Somites.- In vivo Analysis of the Mesenchymal-to-Epithelial Transition during Chick Secondary Neurulation.- Multiscale in vivo Imaging of Collective Cell Migration in Drosophila Embryos.- Methods to Generate Tube Micropatterns for Epithelial Morphogenetic Analyses and Tissue Engineering.- CAFs and Cancer Cells Co-Migration in 3D Spheroid Invasion Assay.- Using Xenopus Neural Crest Explants to Study Epithelial-mesenchymal Transition.- Xenopus Deep Cell Aggregates: a 3D Tissue Model for Mesenchymal-To-Epithelial Transition.- Molecular Tension Microscopy of E-cadherin during Epithelial-Mesenchymal Transition.- Methodologies for Following EMT in vivo at Single Cell Resolution.- Isolation and Identification of EMT Subtypes.- Studying the Metabolism of Epithelial-Mesenchymal Plasticity using the Seahorse XFe96 Extracellular Flux Analyzer.- Inducing Sequential Cycles of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transitions in Mammary Epithelial Cells.- Analysis of cellular EMT States using Molecular Biology and High Resolution FISH Labeling.- Mathematical Modeling of Plasticity and Heterogeneity in EMT.- Control of Cell Migration Using Optogenetics.  

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions