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This dissertation, The Effects of Astrocytic Endothelin-1 in Modulating Inflammatory Pain Processing by Samantha M Y, Chen, 陳雯英, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract:  Endothelin-1 (ET-1), a 21-amino acid peptide, exerts multiple functions including vasoconstriction, neurotransmission and neuromodulation. The correlation between ET-1 and pain was examined by localized injection into the animal showing that pain response was induced in the peripheral nervous system, but reduced in the central nervous system. In addition, the pathogenic effect of ET-1 in the nervous system was found to be cell-type specific as ET-1 expression was significantly induced in the astrocytes under certain pathological conditions such as stroke and Alzheimer's disease. Therefore, in this study, it aims to determine the role of astrocytic ET-1 in inflammatory pain. To better illustrate such notion, transgenic mouse line over-expressing ET-1 in the astrocytes (GET-1) was generated. Under physiological conditions in GET-1 mice, it was revealed that its gross anatomy, in addition with thermal heat and mechanical pain-thresholds, were indistinguishable between GET-1 and its age-matched non-transgenic control (NTg). Inflammatory pain was then induced in the GET-1 mice and NTg mice by subcutaneous formalin injection. Spontaneous and heat-induced pain response were evaluated and analyzed based on the two phases of pain response (i.e. acute and tonic responses). Our results indicated that GET-1 mice exhibited less inflammatory pain-like response when compared to the NTg control, implicating that astrocytic ET-1 over-expression modulates inflammatory pain response. The molecular mechanism substantiating the reduction in this response may be due to the basal expression of pain-mediators in GET-1 mice. Further results showed that over-expression in astrocytic ET-1 evoke an up-regulation in calcitonin gene-related peptide (CGRP) as well as the glutamate transporter-1 (GLT-1), also known as excitatory amino-acid transporters (EAAT2). In addition, under formalin-induced pathological pain, over-expression in astrocytic ET-1 was found to negatively regulate GLT-1 transcription. These findings elucidate the extent that ET-1 contributes to the inflammatory pain pathway via ET-1 and GLT-1 interactions. Overall, this study not only pointed out the involvement of astrocytic ET-1 in inflammatory pain processing, but it also warrants further investigation into the molecular mechanism and potential therapeutic intervention of ET-1-mediated pain. DOI: 10.5353/th_b4775258 Subjects: EndothelinsAstrocytesPain

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