Overview

A first-time collection of cutting-edge morphologic, permeability, transport, in vitro, and molecular techniques to study the workings of the blood brain barrier in health and disease. Described in step-by-step detail, each readily reproducible method includes an explanation of the theoretical basis of the method, a discussion of its advantages and limitations, notes on avoiding pitfalls, and examples of results obtained using the technqiue. These powerful tools can be used to isolate undiscovered molecules, deepen our understanding of their role in health and disease, and identify targets for future gene therapy and therapeutic intervention.

Full Product Details

Author:   Sukriti Nag
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2003 ed.
Volume:   89
Dimensions:   Width: 17.80cm , Height: 3.20cm , Length: 25.40cm
Weight:   1.373kg
ISBN:  

9781588290731

ISBN 10:   1588290735
Pages:   550
Publication Date:   06 August 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Tissue Techniques.- Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels.- Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry.- Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions.- Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy.- Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy.- Blood-Brain Barrier Permeability Techniques.- Pathophysiology of Blood-Brain Barrier Breakdown.- Pial Vessel Permeability to Tracers Using Cranial Windows.- Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry.- Ultracytochemical Studies of the Compromised Blood-Brain Barrier.- Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier.- Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers.- Blood-Brain Barrier Transport Techniques.- A Review of Blood-Brain Barrier Transport Techniques.- In Situ Brain Perfusion Technique.- Brain Efflux Index Method.- Transport Studies Using Membrane Vesicles.- Drug Transport Studies Using Quantitative Microdialysis.- Single-Pass Dual-Label Indicator Method.- Protein Transport in Cerebral Endothelium.- Transport Across the Choroid Plexuses In Vivo and In Vitro.- In Vitro Techniques.- An Overview of In Vitro Techniques for Blood-Brain Barrier Studies.- Isolation and Characterization of Human Brain Endothelial Cells.- Human Brain Microvessel Endothelial Cell and Leukocyte Interactions.- Development and Characterization of Immortalized Cerebral Endothelial Cell Lines.- Isolation and Characterization of Retinal Endothelial Cells.- Isolation and Characterization of Cerebral Microvascular Pericytes.- Molecular Techniques.- Molecular Biology of the Blood-Brain Barrier.- Blood-Brain Barrier Genomics.- Dynamic In Vitro Model of the Blood-Brain Barrier.- Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells.- mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization.- Proteomics of Brain Endothelium.- Immunoblot Detection of Brain Vascular Proteins.- Immunohistochemical Detection of Endothelial Proteins.- Genetically Altered Mice.- Cerebral Vascular Function in Genetically Altered Mice.- Generation of Transgenic Mice by Pronuclear Injection.- Mouse Genome Modification.

Reviews

An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier. -Doody's Health Science Book Review Journal

An outstanding addition to the field! The book is unique in its organization and ease of reading and use as research resource. In my view, this book will emerge as one of the more cited and critical sources of information for all researchers exploring the nature of the blood-brain barrier. - Doody's Health Science Book Review Journal

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