Overview

Over a decade has elapsed since the last volume in this series was published. At that time we considered that we had comprehensively covered all aspects relating to bile acid chemistry and physiology. However, major strides have been made in our understanding of the physiology and pathophysiology of bile acids, due largely to the great advances which have taken place in analytical technology. As a result, the need to document these advances was felt acutely, and therefore this volume is devoted to methodologies in bile acid analysis and their applications. This volume includes twelve chapters written by prominent scientists in the field of bile acid research. The initial chapter discusses techniques of extraction and isolation of bile acids from biological fluids. It is followed by descriptions of physical methods of analysis and discussions of the way these techniques have been applied in the field of bile acid research. Of practical value is the inclusion of a comprehensive list of spectra obtained for refer­ ences by nuclear magnetic resonance spectroscopy and mass spectrometry . These chapters are followed by reviews of biological methods of immuno­ assay and bioluminescence. Specific applications of these techniques are then addressed in contributions relating to bile acid analysis of tissue, serum, urine, and feces. With this integrated approach we have attempted to provide a volume which represents a comprehensive review of the analytical field of bile acids, while also serving as a useful reference book for those workers involved in bile acid analysis.

Full Product Details

Author:   K.D.R. Setchell ,  David Kritchevsky ,  Padmanabhan P. Nair ,  Padmanabhan P Nair
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   Softcover reprint of the original 1st ed. 1988
Dimensions:   Width: 15.20cm , Height: 3.10cm , Length: 22.90cm
Weight:   0.879kg
ISBN:  

9781461282365

ISBN 10:   1461282365
Pages:   604
Publication Date:   02 October 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1 Techniques for Extraction and Group Separation of Bile Acids.- 1. Introduction.- 2. Extraction.- 3. Purification and Group Separation.- 4. Hydrolysis and Solvolysis.- 5. Conclusions.- References.- 2 High-Performance Liquid Chromatography.- 1. Introduction.- 2. Chromatographic Behavior of Bile Acids.- 3. Detection Methods.- 4. Application of HPLC to Biological Specimens.- 5. Conclusions.- References.- 3 Nuclear Magnetic Resonance.- 1. Introduction: Why Use NMR for Bile Acids?.- 2. Basic Principles of NMR.- 3. Proton NMR of Bile Acids.- 4. 13C NMR of Bile Acids.- 5. Recent Advances in NMR Spectroscopy.- 6. Application of NMR to the Physical Chemistry of Bile Acids.- 7. NMR in the Biosynthesis and Metabolism of Bile Acids.- 8. ConclUding Remarks.- References.- 4 X-Ray Crystallography.- l. Introduction.- 2. X-Ray Diffraction by Single Crystals.- 3. Sample Preparation.- 4. Experimental Measurements.- 5. Structure Determination.- 6. Interpretation of Results.- 7. Pitfalls.- 8. Examples.- References.- 5 Mass Spectrometry of Bile Acids.- 1. Introduction.- 2. Principles and Instrumentation.- 3. Ionization Methods.- 4. Methods of Sample Introduction.- 5. Mass Spectrometric Fragmentation of Bile Acids.- 6. Quantitative Analysis of Bile Acids.- 7. Bile Acids Labeled with Stable Isotopes.- 8. Application of Mass Spectrometry to the Determination of Bile Acids in Biological Fluids.- 9. Compendium of Mass Spectra of Bile Acids.- References.- 6 Immunological Methods for Serum Bile Acid Analysis.- 1. Introduction.- 2. Physicochemical State of Bile Acids in Serum.- 3. Immunoassay.- 4. Preparation of Antigenic Bile Acids.- 5. Antibodies.- 6. Labeled Bile Acids.- 7. Assay Procedure.- 8. Comparison of Methods.- 9. Comparison between Methods.- 10. Enzyme Immunoassay.- 11. Serum Bile Acidsin Physiology and Pathology.- References.- 7 Bioluminescence Assays Using Immobilized Enzymes in Bile Acid Analysis.- 1. Introduction.- 2. Bioluminescence Assays.- 3. Material.- 4. Methods.- 5. Results.- 6. Discussion.- 7. Summary.- References.- 8 Bile Acids in Extrahepatic Tissues.- 1. Introduction.- 2. Analytical Methods.- 3. Applications.- 4. Conclusions.- References.- 9 Tissue-Bound Bile Acids.- 1. Introduction.- 2. Methodology.- References.- 10 Serum Bile Acids.- 1. Introduction.- 2. Serum Bile Acids and the Different Enterohepatic Bile Acid Cycles in Normal Humans.- 3. Serum Bile Acid Measurements and the Dynamics of the Normal Enterohepatic Circulations.- 4. Serum Bile Acids and Liver Disease.- 5. Gas Chromatography-Mass Spectrometry and Monohydroxy Bile Acids in Liver Disease.- 6. Serum Bile Acid Composition in Other Circumstances.- 7. Conclusion.- References.- 11 Urinary Bile Acids.- 1. Introduction.- 2. Methodology.- 3. Bile Acids Detected in the Urine.- 4. Origin of the Atypical Bile Acids in the Urine.- 5. The Renal Clearance of Bile Acids.- 6. Conclusion.- References.- 12 Fecal Bile Acids.- 1. Introduction.- 2. Methods for Determination of Fecal Bile Acids.- 3. Fecal Bile Acid Excretion in Health.- 4. Fecal Bile Acid Excretion in Disease.- 5. Effect of Diet and Drugs on Fecal Bile Acid Excretion.- 6. Conclusions.- References.

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