Overview

The molecular basis of disease is the driving force in biomedical research. Gene detection, quantification, and localization are required to determine disease pathogenesis. In as much as DNA is the genetic potential in a particular organism, RNA and protein expression are the functional result of this genetic programme. This book describes methods to quantify and localize gene expression, with a particular emphasis on the sensitive detection of low abundance gene expression. Sensitive detection of gene expression has been hindered by poor recovery or preservation of target sequences. The text describes optimized methods to ensure full recovery and preservation of targets from specimen acquisition through detection of specific targets and final analysis. This text also focuses upon in situ analysis of cells and tissues

Full Product Details

Author:   Bruce K. Patterson
Publisher:   Birkhauser Boston Inc
Imprint:   Birkhauser Boston Inc
Edition:   2000 ed.
Dimensions:   Width: 15.50cm , Height: 1.20cm , Length: 23.50cm
Weight:   0.430kg
ISBN:  

9780817640347

ISBN 10:   0817640347
Pages:   150
Publication Date:   24 January 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Basic Methods.- 1. Basic Flow Cytometry Instrumentation.- 2. Fixation for In Situ Molecular Analysis.- 3. A Method for Reduction of Green Wavelength Autofluorescence Emission in Cellular Preparations.- 4. Gene Quantification: Choosing the Target.- 5. Detection and Quantification of Cytokine-Producing Cells by Immunostaining.- 6. In Situ Hybridization Using the bDNA Technology.- Specific Applications.- 7. Kaposi’s Sarcoma—Associated Human Herpes Virus Eight: Localization of Viral Gene Expression and Viral Latency.- 8. Pathogenetic Mechanisms of Animal Orbiviruses That Cause Disease at Low Copy Number.- 9. Localization of Hepatitis C Virus Using Reverse Transcriptase Fluorescence In Situ 5’ Nuclease Assay.- 10. Simultaneous Quantification and Localization of HIV gag-pol Expression Using Ultrasensitive Fluorescence In Situ Hybridization.- 11. Quantification and Localization of Gene Expression: Future Directions.

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