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OverviewFull Product DetailsAuthor: Gennaro De LiberoPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 2nd ed. 2009 Volume: 514 Weight: 0.466kg ISBN: 9781617376610ISBN 10: 1617376612 Pages: 166 Publication Date: 10 January 2011 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Out of stock ![]() The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available. Table of ContentsAnalysis of Frequency and Phenotype of Antigen-Specific T Cells.- B Cell Helper Assays.- transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing.- Flow Cytometry and Cell Activation.- Investigating T Cells by Polychromatic Flow Cytometry.- Generation of Human T Cell Clones.- Limiting Dilution Analysis of Antigen-Specific T Cells.- T Cell Epitope-Mapping by Cytokine Gene Expression Assay.- Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications.- Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE.- Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation.ReviewsFrom the reviews of the second edition: A great collection of protocols, some totally new and some upgraded ! to study the biology of this fascinating cell is wellcome by the scientific community. ! Thanks to the generation of ! PFC, is now at the hands of those scientists that want to advance our understanding of T cells biology. ! the book will be of enourmous help both to beginners and to experienced scientists is well underlining the esprit of the book, as I showed with the three examples illustrated. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009) From the reviews of the second edition: A great collection of protocols, some totally new and some upgraded ... to study the biology of this fascinating cell is wellcome by the scientific community. ... Thanks to the generation of ... PFC, is now at the hands of those scientists that want to advance our understanding of T cells biology. ... the book will be of enourmous help both to beginners and to experienced scientists is well underlining the esprit of the book, as I showed with the three examples illustrated. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009) Author InformationTab Content 6Author Website:Countries AvailableAll regions |