Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences

Author:   Robert E. Rhoads
Publisher:   Humana Press Inc.
Edition:   1st ed. 2016
Volume:   1428
ISBN:  

9781493936236


Pages:   320
Publication Date:   29 May 2016
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences


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Author:   Robert E. Rhoads
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2016
Volume:   1428
Dimensions:   Width: 17.80cm , Height: 1.90cm , Length: 25.40cm
Weight:   7.684kg
ISBN:  

9781493936236


ISBN 10:   1493936239
Pages:   320
Publication Date:   29 May 2016
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences.- Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments.- Enzymatic Modification of 5’-Capped RNA and Subsequent Labeling by Click Chemistry.- Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG.- Intronless b-Globin Reporter:  A Tool for Studying Nuclear RNA Stability Elements.- Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA.- Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells.- Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System.- GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use.- Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems.- FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines.- Transfecting Human Monocytes with RNA.- In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated byPiggyBac Transposition.- Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.- Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.- Immune Monitoring using mRNA-Transfected Dendritic Cells.- Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2.- mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70).- Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation.- Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA.- Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA.

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