Overview

This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Filippo Menolascina
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2229
Weight:   0.692kg
ISBN:  

9781071610343

ISBN 10:   1071610341
Pages:   353
Publication Date:   07 January 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Qualitative Modeling, Analysis and Control of Synthetic Regulatory Circuits Madalena Chaves and Hidde de Jong   2. Stochastic Differential Equations for Practical Simulation of Gene Circuits Jesus Pico, Alejandro Vignoni, and Yadira Boada   3. Using Models To (Re-)Design Synthetic Circuits Giselle McCallum and Laurent Potvin-Trottier   4. Automated bio-circuit design with SYNBADm Irene Otero-Muras and Julio R. Banga   5. Setting up an Automated Biomanufacturing Laboratory Marilene Pavan   6. Computer-aided Design and Pre-validation of Large Batches of DNA Assemblies Valentin Zulkower   7. Computer-aided Planning for the Verification of Large Batches of DNA Constructs Valentin Zulkower   8. Characterizing Genetic Parts and Devices using RNA Sequencing Deepti Vipin, Zoya Ignatova, and Thomas E. Gorochowski   9. Steady-State Cell-Free Gene Expression with Microfluidic Chemostats Nadanai Laohakunakorn, Barbora Lavickova, Zoe Swank, Julie Laurent, and Sebastian J. Maerkl   10. A Microfluidic/Microscopy-Based Platform for On-Chip Controlled Gene Expression in Mammalian Cells Mahmoud Khazim, Elisa Pedone, Lorena Postiglione, Diego di Bernardo, and Lucia Marucci   11. Optimal Experimental Design for Systems and Synthetic Biology Using Amigo2 Eva Balsa-Canto, Lucia Bandiera, and Filippo Menolaschina   12. A Cyber-Physical Platform for Model Calibration Lucia Bandiera, David Gomez Cabeza, Eva Balsa-Canto, and Filippo Menolascina   13. Prediction of cellular burden with host-circuit models Evangelos-Marios Nikolados, Andrea Y. Weiße, and Diego A. Oyarz´un   14. A Practical Step-by-Step Guide for Quantifying Retroactivity in Gene Networks Andras Gyorgy   15. Engineering Sensors for Gene Expression Burden Alice Boo and Francesca Ceroni   16. Engineering Protein-based Parts for Genetic Devices in Mammalian Cells Giuliano Bonfa, Federica Cella, and Velia Siciliano

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions