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Author:   Helle Ulrich
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2009 ed.
Volume:   497
Dimensions:   Width: 19.30cm , Height: 2.30cm , Length: 26.00cm
Weight:   0.921kg
ISBN:  

9781934115800

ISBN 10:   1934115800
Pages:   324
Publication Date:   23 October 2008
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The SUMO System: An Overview.- Identification of Sumo Targets.- Identification of SUMO Target Proteins by Quantitative Proteomics.- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry.- Identification of SUMO Targets Through In Vitro Expression Cloning.- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS).- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae.- Identification Of Sumo Interactors.- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis.- Identification of SUMO-Binding Motifs by NMR.- Functional Analysis Of Sumoylation In Vivo.- Regulation of Transcription Factor Activity by SUMO Modification.- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization.- Biochemical Studies Of Sumo Conjugation.- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation.- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes.- Preparation of Sumoylated Substrates for Biochemical Analysis.- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli.- Biochemical Studies of Sumo Deconjugation.- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates.- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage.- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities.- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay.- Manipulation And Alternative Uses Of The Sumo System.- Inhibition of the SUMO Pathway by Gam1.- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.

Reviews

From the reviews: The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques ! . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field ! and regulated by SUMO. ! for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves. (Peter O'Hare, Microbiology Today, July, 2009)

From the reviews: The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques ... . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field ... and regulated by SUMO. ... for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves. (Peter O'Hare, Microbiology Today, July, 2009)

From the reviews: The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field and regulated by SUMO. for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves. (Peter O Hare, Microbiology Today, July, 2009)

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