Overview

This book examines detailed experimental and computational approaches for the analysis of many aspects vital to the understanding of membrane protein structure and function. Readers will receive guidance on the selection and use of methods for over-expression and purification, tools to characterize membrane proteins within different phospholipid bilayers, direction on functional studies, and approaches to determine the structures of membrane proteins. Detailed experimental steps for specific membrane proteins with critical notes allow the protocols to be modified to different systems. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of practical information and implementation advice that leads to excellent, reproducible results.  Authoritative and up-to-date, Structure and Function Studies of Membrane Proteins serves as an ideal guide for biologists, biochemists, and biophysicists striving to furtherunderstand these essential proteins and their many biological roles.

Full Product Details

Author:   Ingeborg Schmidt-Krey ,  James C. Gumbart
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2302
Weight:   0.706kg
ISBN:  

9781071613962

ISBN 10:   1071613960
Pages:   358
Publication Date:   21 April 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Expression and Purification of Human Mitochondrial Intramembrane Protease PARL             Elena Arutyunova, Laine Lysyk, Melissa Morrison, Cory Brooks, and M. Joanne Lemieux   2. Reconstitution of Detergent-Solubilized Membrane Proteins into Proteoliposomes and Nanodiscs for Functional and Structural Studies             Kerry M. Strickland, Kasahun Neselu, Arshay J. Grant, Carolann L. Espy, Nael A. McCarty, and Ingeborg Schmidt-Krey   3. Biochemical Characterization of GPCR–G Protein Complex Formation             Filip Pamula and Ching-Ju Tsai   4. Electrophysiological Approaches for the Study of Ion Channel Function             Guiying Cui, Kirsten A. Cottrill, and Nael A. McCarty   5. Isothermal Titration Calorimetry of Membrane Proteins             Han N. Vu, Alan J. Situ, and Tobias S. Ulmer   6. Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level             Kanokporn Chattrakun, Katherine G. Schaefer, Lucas S. Chandler, Brendan P. Marsh, and Gavin M. King   7. Structure Determination of Membrane Proteins Using X-Ray Crystallography             Evan Billings, Karl Lundquist, Claire Overly, Karthik Srinivasan, and Nicholas Noinaj   8. Studying Membrane Protein Structures by MicroED             Michael W. Martynowycz and Tamir Gonen   9. Single-Particle Cryo-EM of Membrane Proteins             Dovile Januliene and Arne Moeller   10. Helical Membrane Protein Crystallization in the New Era of Electron Cryo-Microscopy             Mary D. Hernando, Joseph O. Primeau, and Howard S. Young   11. NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel             Robert Silvers and Matthew Eddy   12. Preparation of a Deuterated Membrane Protein for Small-Angle Neutron Scattering             Yuqi Wu, Kevin L. Weiss, and Raquel L. Lieberman   13. Preparing Membrane Proteins for Simulation Using CHARMM-GUI             Yupeng Li, Jinchan Liu, and James C. Gumbart   14. Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins: A Practical Guide             William Glass, Jonathan W. Essex, Franca Fraternali, James Gebbie-Rayet, Irene Marzuoli, Marley L. Samways, Philip C. Biggin, and Syma Khalid   15. Continuous Constant pH Molecular Dynamics Simulations of Transmembrane Proteins             Yandong Huang, Jack A. Henderson, and Jana Shen   16. Molecular Dynamics-Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions             Dylan Ogden and Mahmoud Moradi   17. Concepts, Practices, and Interactive Tutorial for Allosteric Network Analysis of Molecular Dynamics Simulations             Wesley M. Botello-Smith and Yun Lyna Luo   18. Large Scale Molecular Dynamics Simulations of Cellular Compartments             Eric Wilson, John Vant, Jacob Layton, Ryan Boyd, Hyungro Lee, Matteo Turilli, Benjamín Hernández, Sean Wilkinson, Shantenu Jha, Chitrak Gupta, Daipayan Sarkar, and Abhishek Singharoy

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