Sphingosine-1-Phosphate: Methods and Protocols

Author:   Alice Pébay ,  Kursad Turksen
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2018
Volume:   1697
ISBN:  

9781493984787


Pages:   182
Publication Date:   30 August 2018
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Sphingosine-1-Phosphate: Methods and Protocols


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Author:   Alice Pébay ,  Kursad Turksen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2018
Volume:   1697
Weight:   0.454kg
ISBN:  

9781493984787


ISBN 10:   1493984780
Pages:   182
Publication Date:   30 August 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay.- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity.- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells.- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry.- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines.- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule.- A Rapid Fluorescence Assayfor Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes.- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry.- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses.- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate.- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor.- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors.- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering.

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