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Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  Alfred H. Merrill Jr. (Emory University, Atlanta, Georgia, U.S.A.) ,  Yusuf A. Hannun (Medical University of South Carolina, Charleston, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 312
Dimensions:   Width: 15.20cm , Height: 4.10cm , Length: 22.90cm
Weight:   1.040kg
ISBN:  

9780121822132

ISBN 10:   0121822133
Pages:   688
Publication Date:   25 October 2000
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Section I: Methods for Analyzing Sphingolipids [1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography [2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate [3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography [4]: Quantitative Determination of Ceramide Using Diglyceride Kinase [5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry [6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography [7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography [8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample [9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges [10]: Ganglioside Analysis by High-Performance Thin-Layer Chromatography [11]: Purification and Analysis of Gangliosides [12]: Thin-Layer Chromatography Blotting Using Polyvinylidene Difluoride Membrane (Far-Eastern Blotting) and Its Applications [13]: Thin-Layer Chromatography Immunostaining [14]: Monoclonal Anti-Glycosphingolipid Antibodies [15]: Immunolocalization of Gangliosides by Light Microscopy Using Anti-Ganglioside Antibodies [16]: Cloud-Point Extraction of Gangliosides using Nonionic Detergent C14EO6 [17]: Analyses of Glycosphingolipids Using Clam, Mercenaria mercenaria, Ceramide Glycanase [18]: Quantitative Analyses of Binding Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance [19]: Use of Circular Dichroism for Assigning Stereochemistry of Sphingosine and Other Long-Chain Bases [20]: Infrared Determination of Conformational Order and Phase Behavior in Ceramides and Stratum Corneum Models [21]: Use of Nuclear Magnetic Resonance Spectroscopy in Evaluation of Ganglioside Structure, Conformation, and Dynamics [22]: Fluorescence Quenching Assay of Sphingolipid/Phospholipid Phase Separation in Model Membranes Section II: Methods for Analyzing Aspects of Sphingolipid Metabolism in Intact Cells [23]: Synthesis of Fluorescent Substrates and Their Application to Study of Sphingolipid Metabolism in Vitro and in Intact Cells [24]: Selection of Mammalian Cell Mutants in Sphingolipid Biosynthesis [25]: Selection of Yeast Mutants in Sphingolipid Metabolism [26]: Fluorescence-Based Selection of Gene-Corrected Hematopoietic Stem and Progenitor Cells Based on Acid Sphingomyelinase Expression [27]: Mammalian Ganglioside Sialidases: Preparation and Activity Assays Section III: Sphingolipid–Protein Interactions and Cellular Targets [28]: Effects of Sphingosine and Other Sphingolipids on Protein Kinase C [29]: Kinetic Analysis of Sphingoid Base Inhibition of Yeast Phosphatidate Phosphatase [30]: Assays of Sphingosine-Dependent Kinase for 14-3-3 Protein [31]: Synthesis and Use of Caged Sphingolipids [32]: Binding of Sphingosine 1-Phosphate to Cell Surface Receptors [33]: Use of Short-Chain Ceramides [34]: Analysis of Ceramide-Activated Protein Phosphatases [35]: Use of Affinity Chromatography and TID-Ceramide Photoaffinity Labeling for Detection of Ceramide-Binding Proteins [36]: Lectin-Mediated Cell Adhesion to Immobilized Glycosphingolipids [37]: Analysis of Glycolipid-Dependent Cell Adhesion Based on Carbohydrate–Carbohydrate Interaction [38]: Analysis of Interactions between Glycosphingolipids and Microbial Toxins [39]: Oxidation of Aglycone of Glycosphingolipids: Serine and Ceramide Acid Precursors for Soluble Glycoconjugates [40]: Separation of Glycosphingolipid-Enriched Microdomains from Caveolar Membrane Characterized by Presence of Caveolin [41]: Reconstitution of Sphingolipid–Cholesterol Plasma Membrane Mlcrodomalns for Studies of Virus-Glycolipid Interactions [42]: Analysis of Ceramides Present in Glycosylphosphatidylinositol Anchored Proteins of Saccharomyces cerevisiae [43]: Preparation of Functionalized Lipid Tubules for Electron Crystallography of Macromolecules Section IV: Sphingolipid Transport and Trafficking [44]: Applications of BODIPY–Sphingolipid Analogs to Study Lipid Traffic and Metabolism in Cells [45]: Using Biotinylated Gangliosides to Study Their Distribution and Traffic in Cells by Immunoelectron Microscopy [46]: Assays for Transmembrane Movement of Sphingolipids Section V: Other Methods [47]: Compilation of Methods Published in Previous Volumes of Methods in Enzymology Author Index Subject Index

Reviews

Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

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