Overview

This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.

Full Product Details

Author:   Marcelo Tigre Moura
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2647
Weight:   0.831kg
ISBN:  

9781071630631

ISBN 10:   1071630636
Pages:   320
Publication Date:   12 April 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.    Cloning by SCNT: integrating technical and biology-driven advances Marcelo T. Moura   2.    Epigenetic reprogramming and somatic cell nuclear transfer             Luna N. Vargas, Márcia M. Silveira and Maurício M. Franco   3.    Early cell specification in mammalian fertilized and somatic cell nuclear transfer embryos             Marcelo D. Goitis and Jose B. Cibelli   4.    Mitochondrial inheritance following nuclear transfer: from cloned animals to patients with mitochondrial disease             Jörg P. Burgstaller and Marcos R. Chiaratti   5.    Stem cells as nuclear donors for mammalian cloning             Carolina G. Silva and Carlos F. Martins   6.    Animal Transgenesis and cloning: combined development and future perspectives             Melissa S. Yamashita and Eduardo O. Melo             7.    Mouse cloning using outbred oocyte donors and non-toxic reagents             Sayaka Wakayama, Yukari Terashita, Yoshiaki Tanabe, Naoki Hirose and Teruhiko Wakayama   8.    Somatic cell nuclear transfer in rabbits             Pengxiang Qu, Wenbin Cao and Enqi Liu   9.    Production of cloned pigs by handmade cloning             Gábor Vajta, Wen B. Chen and Zoltan Machaty   10.  Somatic Cell Nuclear Transfer in Pigs             Werner G. Glanzner, Vitor B. Rissi and Vilceu Bordignon   11.  Somatic cell nuclear transfer using freeze-dried protaminized donor nuclei             Luca Palazzese, Marta Czernik, Kazutsugu Matsukawa and Pasqualino Loi   12.  Cattle cloning by somatic cell nuclear transfer             Juliano R. Sangalli, Rafael V. Sampaio, Tiago H. C. De Bem, Lawrence C. Smith and Flávio V. Meirelles   13.  Production of water buffalo SCNT embryos by handmade cloning             Prabhat Palta, Naresh L. Selokar and Manmohan S. Chauhan   14.  Bovid interspecies somatic cell nuclear transfer with ooplasm transfer             Antonio L. González-Grajéales and Gabriela F. Mastromonaco   15.  Horse somatic cell nuclear transfer using zona pellucida intact and zona-free oocytes             Daniel Salamone and Marc Maserati   16.  A modified handmade cloning method for dromedary camels             Fariba Moulavi and Sayyed M. Hosseini   17.  Derivation of bovine primed embryonic stem cells from somatic cell nuclear transfer embryos             Delia A. Soto, Micaela Navarro and Pablo J. Ross

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