Skeletal Development and Repair: Methods and Protocols

Author:   Matthew J. Hilton
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1130
ISBN:  

9781493963102


Pages:   328
Publication Date:   23 August 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Skeletal Development and Repair: Methods and Protocols


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Author:   Matthew J. Hilton
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1130
Dimensions:   Width: 17.80cm , Height: 1.80cm , Length: 25.40cm
Weight:   6.467kg
ISBN:  

9781493963102


ISBN 10:   1493963104
Pages:   328
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Overview of Skeletal Development.- Overview of Skeletal Repair (Fracture Healing and Its Assessment).- Generation of Closed Transverse Fractures in Small Animals.- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool.- Surgical Induction of Posttraumatic Osteoarthirits.- A Mouse Model of Flexor Tendon Repair.- Cartilage Explant Cultures.- Renal Capsule Transplantations to Assay Skeletal Angiogenesis.- Whole Mount Skeletal Staining.- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens.- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012).- Demineralized Murine Skeletal Histology.- Beta-Galactosidase Staining in the Skeleton.- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues.- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections.- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections.- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections.- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis.- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme.- Isolation and Culture of Murine Primary Chondrocytes.- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo.- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts.- RANKL-BasedOsteoclastogenic Assays from Murine Bone Marrow Cells.- Hematopoietic Stem Cell Cultures and Assays.

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