Overview

This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

Full Product Details

Author:   Nikolai Eroshenko
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1642
Dimensions:   Width: 17.80cm , Height: 2.00cm , Length: 25.40cm
Weight:   6.685kg
ISBN:  

9781493984053

ISBN 10:   1493984055
Pages:   342
Publication Date:   12 May 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Generating Genetically Modified Mice: A Decision Guide.- Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes.- Building Cre Knockin Rat Lines using CRISPR/Cas9.- Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases.- Use of the DICE Dual Integrase Cassette Exchange System.- Ligand-controlled Site-specific Recombination in Zebrafish.- Injection-based Delivery of Cell Permeable Peptide-tagged Cre.- Viral Delivery of GFP-dependent Recombinases to the Mouse Brain.- Recombinase-mediated Cassette Exchange using Adenoviral Vectors.- Marker Removal in Transgenic Plants using Cre recombinase Delivered with Potato Virus X.- Nanoparticle-mediated Recombinase Delivery into Maize.- Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines.- FLPing Genes On and Off in Drosophila.- Imaging Neural Architecture in Brainbow Samples.- Purification and in vitro characterization.

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