Overview

This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.

Full Product Details

Author:   Aik T. Ooi
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2386
Weight:   0.625kg
ISBN:  

9781071617731

ISBN 10:   1071617737
Pages:   311
Publication Date:   13 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections             Preethi Vijayaraj   2. Indirect Immunofluorescence of Tissue Sections             Cody J. Aros   3. Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique             Valentina Proserpio, Laura Conti, and Salvatore Oliviero   4. High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry             Marco A. Fernandez, Hammad Alzayat, Maria C. Jaimes, Yacine Kharraz, Gerard Requena, and Pedro Mendez   5. Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot)             Bernt Axelsson   6. Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot             Bernt Axelsson   7. Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing             Samuel C. Kim, John R. Haliburton, Zev J. Gartner, and Adam R. Abate   8. Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis             Ákos Végvári, Jimmy E. Rodrigues, and Roman A. Zubarev   9. Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels             Carlene Petes, Stephen K.H. Li, Shariq Mujib, Michelle M. Poulin, Noah Saederup, Andrew A. Quong, and Christina Loh   10. High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging             Ofer Elhanani, Leeat Keren, and Michael Angelo   11. Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA             Jing Lin and Savaş Tay   12. High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform             David W. Ruff, Dalia Dhingra, Kathryn Thompson, Jacqueline A. Marin, and Aik T. Ooi   13. Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform             Nicole Mende, Elisa Laurenti, Berthold Gottgens, and Nicola K. Wilson   14. Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets             Benjamin Lacar   15. Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits             Mandi Wong, Carol Kosman, Liane Takahashi, and Naveen Ramalingam   16. Combined Measurement of RNA and Protein Expression on a Single-Cell Level             Valentina Russo, Nadia Brasu, and Luigia Pace   17. Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities             Dalia Dhingra, Aik T. Ooi, and David W. Ruff

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