Single Cell Analysis: Methods and Protocols

Author:   Miodrag Gužvić
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2752
ISBN:  

9781071636206


Pages:   260
Publication Date:   10 January 2024
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Single Cell Analysis: Methods and Protocols


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Author:   Miodrag Gužvić
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2752
Weight:   0.722kg
ISBN:  

9781071636206


ISBN 10:   1071636200
Pages:   260
Publication Date:   10 January 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Acknowledgement…Preface…Table of Contents…Contributing Authors… 1. Single Cell Isolation from Surgically Resected Tissue via Mechanical Dissociation using TissueGrinderPrama Pallavi and Stefan Scheuermann 2. Circulating Tumor Cell Enrichment and Single Cell Isolation Combining the CellSearch® and DEPArray™ SystemsCäcilia Köstler, Bernhard Polzer, and Barbara Alberter 3. Isolation of Viable Epithelial and Mesenchymal Circulating Tumor Cells from Breast Cancer PatientsJustyna Topa, Anna J. Żaczek, and Aleksandra Markiewicz 4. Single Cell Recovery from Tumor Cell Xenotransplanted Zebrafish Embryos for the Study of Metastasis Initiating CellsPablo Hurtado, Inés Martínez-Pena, and Roberto Piñeiro 5. Isolation of Single Circulating Tumor Cells using VyCAP Puncher SystemThais Pereira-Veiga, Bianca Behrens, Joska J. Broekmaat, Lisa Oomens, Michiel Stevens, Arjan G.J. Tibbe, Nikolas Stoecklein, Laura Muinelo-Romay, Roberto Piñeiro, and Clotilde Costa 6. Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single CellMiodrag Gužvić 7. Isolation and Genomic Analysis of Circulating Tumor Cell Clusters in Cancer PatientsCarolina Reduzzi, Marta Vismara, Thomas Schamberger, Marco Silvestri, Rosita Motta, Bernhard M. Polzer, and Vera Cappelletti 8. Establishing Single Cell Clones from In Vitro Cultured Circulating Tumor CellsTeng Teng and Min Yu 9. Immunofluorescence Combined with Single-Molecule RNA Fluorescence In Situ Hybridization for Concurrent Detection of Proteins and Transcripts in Stress GranulesJakub Kochan and Mateusz Wawro 10. Highly Multiplexed and Simultaneous Characterization of Protein and RNA in Single Cells by Flow or Mass Cytometry Platforms using Proximity Ligation Assay for RNAAndrew D. Duckworth, Joseph R. Slupsky, and Nagesh Kalakonda 11. Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single CellsGiancarlo Feliciello, Zbigniew Tadeusz Czyz, and Bernhard Polzer 12. Single Cell MicroRNA Sequencing Library PreparationSarah M. Hücker and Stefan Kirsch 13. Immunoblot Analysis from Single Cells using Milo™ Single-Cell Western PlatformPrashant V. Thakkar 14. Imaging of Sub-Cellular Distribution of Platinum in Single Cells using Laser Ablation Inductively Coupled Plasma Mass SpectrometryAmy J. Managh and Calum Greenhalgh 15. Patch-seq: Multimodal Profiling of Single-Cell Morphology, Electrophysiology, and Gene ExpressionCathryn R. Cadwell and Andreas S. Tolias 16. Single-Nucleus ATAC-seq for Mapping Chromatin Accessibility in Individual Cells of Murine HeartsMichail Yekelchyk, Xiang Li, Stefan Guenther, and Thomas Braun Subject Index List…

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