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OverviewThis detailed volume explores the most recent methodologies and applications of stable isotope labeling by amino acids in cell culture (SILAC). With the development of new technologies in the field of mass spectrometry and bioinformatics, SILAC-based methods have become the first tool of choice in quantitative proteomics either on its own or in combination with other more recently developed approaches, which is elucidated in the chapters of this book. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, SILAC: Methods and Protocols serves as an ideal guide for researchers working in the area of functional proteomics and other aspects of protein science. Full Product DetailsAuthor: Jose L. Luque-GarciaPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2023 Volume: 2603 Weight: 0.765kg ISBN: 9781071628621ISBN 10: 1071628623 Pages: 286 Publication Date: 13 November 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand ![]() We will order this item for you from a manufactured on demand supplier. Table of Contents1. Dynamic SILAC to Determine Protein Turnover in Neurons and Glia Aline R. Dörrbaum, Erin M. Schuman, and Julian D. Langer 2. SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe Dorothea Anrather, Silvia Bagelova Polakova, Lubos Cipak, and Juraj Gregan 3. Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics Monika Kopeckova, Marek Link, and Ivona Pavkova 4. pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles So-Fong Cam Ngan, Neil E. McCarthy, and Siu Kwan Sze 5. Quantification of Protein Palmitoylation by Cysteine-SILAC Caiyun Fang, Xiaoqin Zhang, and Haojie Lu 6. Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC) Georgia Chachami and Sina-Victoria Barysch 7. A Super-SILAC Approach for Profiling Histone Post-Translational Modifications Roberta Noberini, Elisa Longhi, and Tiziana Bonaldi 8. SILAC-Based Quantitative Phosphoproteomics in Yeast María Luisa Hernáez and Concha Gil 9. Phosphotyrosine Profiling Using SILAC Keshava K. Datta, Aditi Chatterjee, and Harsha Gowda 10. Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins Rong Cai and Yinsheng Wang 11. Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules Tomonori Tamura and Itaru Hamachi 12. Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC Alexis Gonneaud, Claude Asselin, Véronique Giroux, and François-Michel Boisvert 13. Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons Guoan Zhang, Katrin Deinhardt, and Thomas A. Neubert 14. Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry Enrico Massignani, Marianna Maniaci, and Tiziana Bonaldi 15. SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos Alistair Langlands, Hamze Beati, and H.- Arno J. Müller 16. Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae Guillermo Aragoneses-Cazorla, Andres Machuca, M. Pilar Buendia-Nacarino, Daniela S. Anunciação, Estefania Garcia-Calvo, and Jose L. Luque-Garcia 17. Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC Takashi Kubota 18. Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions Jolene M. Duda and Stefani N. Thomas 19. Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC Jihye Shin and Cheolju Lee 20. Using SILAC to Develop Quantitative Data Independent Acquisition (DIA) Proteomics Methods Ellen P. Casavant, Jason Liang, Sumedh Sankhe, W. Rodney Mathews, and Veronica G. Anania 21. SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status Marie Vajrychova, Barbora Salovska, Kristyna Pimkova, Ivo Fabrik, and Zdenek Hodny 22. Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-Length Protein Calibrators (TAQSI) Xinwen Wang, Jian Shi, and Hao-Jie ZhuReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |