Overview

This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.

Full Product Details

Author:   Alexander E. Kalyuzhny
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2023
Volume:   2593
Weight:   0.838kg
ISBN:  

9781071628102

ISBN 10:   1071628100
Pages:   328
Publication Date:   14 December 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Top 5 Considerations When Choosing an Antibody             Amy Archuleta and Michael D. Browning   2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting             Biji T. Kurien and R. Hal Scofield   3. Recent Advances in Chromogens for Immunohistochemistry             Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key   4. Overview of Imaging Techniques in Immunohistochemistry             Gerald Sedgewick   5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells             Pravin J. Mishra and Debabrata Banerjee   6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments             Hemant K. Mishra   7. Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining             Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas   8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species             Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz   9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes             Sharla L. White, Amy T. Lam, and Hannah D. Buck   10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research             Simeon C. Daeschler and Gregory H. Borschel   11. Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach             Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski   12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing             Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier   13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells             Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny   14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue             Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny   15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry             Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova   16. Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells             Junjiao Wu and Yu Tang   17. Immunohistochemical Techniques for Phosphoproteins             Berenice L. Sanz Ressel and Alfredo A. Molinolo   18. Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells             Laurie S. Minamide, Ryan Hylton, Matthew Swulius, and James R. Bamburg   19. Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections             Clara Muñoz-Castro, Ayush Noori, Bradley T. Hyman, and Alberto Serrano-Pozo   20. Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry             Lokman Pang, Matthias Ernst, and Jennifer Huynh   21. Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometryTM             Thomas D. Campbell, Arne Christians, and Madison A. Tyler

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