Overview

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab.  Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and thenovel coronavirus disease that results from it.

Full Product Details

Author:   Justin Jang Hann Chu ,  Bintou Ahmadou Ahidjo ,  Chee Keng Mok
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2452
Weight:   1.121kg
ISBN:  

9781071621103

ISBN 10:   1071621106
Pages:   478
Publication Date:   14 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Clinical and Diagnostic Virology   1. Evolution and Epidemiology of SARS-CoV-2 Virus             Yu-Nong Gong, Kuo-Ming Lee, and Shin-Ru Shih   2. Molecular Epidemiology of SARS-CoV-2 by Sequencing             Yan Yan and Qinxue Hu   3. Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2             Xuemei Yang, Ning Dong, and Sheng Chen   4. Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2:  Assessing Performance             Vidya Keshav, Lesley Scott, Anura David, Lara Noble, Elizabeth Mayne, and Wendy Stevens   5. Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens             Yasuo Suda, Yasuhisa Tajima, Jun-ichiro Nishi, and Takashi Kajiya   6. Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay             Alexander Wilhelm, Christiane Pallas, Rolf Marschalek, and Marek Widera   7. Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans             Linda Hueston   Part II: Cell Culture, Virus Replication, and Cellular Responses   8. Propagation and Quantification of SARS-CoV-2             Alexander S. Jureka and Christopher F. Basler   9. Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay             C. Korin Bullen, Stephanie L. Davis, and Monika M. Looney   10. One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification             Raphael Nyaruaba, Xiohong Li, Caroline Mwaliko, Faith Ogolla, Changchang Li, Lu Zhao, Hang Yang, Junping Yu, and Honping Wei   11. Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics             Lucia Grenga, Duarte Gouveia, and Jean Armengaud   12. Detection of SARS-CoV-2 by Mass Spectrometry             Petra Wandernoth, Katharina Kriegsmann, Jörg Kriegsmann, and Mark Kriegsmann   13. Bioinformatics-Based Approaches to Study Virus–Host Interactions During SARS-CoV-2 Infection             Muhammad Saad Khan, QudsiaYousafi, Shabana Bibi, Muhammad Azhar, and Awais Ihsan   14. Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2             Kai Sen Tan, Akshamal M. Gamage, and Jing Liu   Part III: Immunology and Animal Models   15. Animal Models of COVID-19: Nonhuman Primates             Dhiraj Kumar Singh, Journey Cole, Ruby Escobedo, Kendra J. Alfson, Bindu Singh, Tae-Hyung Lee, Xavier Alvarez, Shashank R. Ganatra, Ricardo Carrion, Jr, and Deepak Kaushal   16. Animal Models of COVID-19: Transgenic Mouse Model             Jun-Gyu Park, Paula A. Pino, Anwari Akhter, Xavier Alvarez, Jordi B. Torrelles, and Luis Martinez-Sobrido   17. Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry             Silvia Lonardi, Mattia Bugatti, Arianna Valzelli, and Fabio Facchetti   18. Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses             Sabari Nath Neerukonda, Russell Vassell, Carol D. Weiss, and Wei Wang   Part IV: Antivirals and Vaccines   19. Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach             Erica T. Prates, Michael R. Garvin, Piet Jones, J. Izaak Miller, Kyle A. Sullivan, Ashley Cliff, Joao Gabriel Felipe Machado Gazolla, Manesh Shah, Angelica M. Walker, Matthew Lane, Christopher T. Rentsch, Amy Justice, Mirko Pavicic, Jonathon Romero, and Daniel Jacobson   20. Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test             Sandra Westhaus and Holger F. Rabenau   21. Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2             Corey Balinsky, Vihasi Jani, Peifang Sun, Maya Williams, Gabriel Defang, and Kevin R. Porter   22. Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2             Yan Ling Ng, Chee-Keng Mok, and Justin Jang Hann Chu   Part V: Biorisk and Mitigation Measurements   23. Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2             Sabai Phyu, Tessy Joseph, and Margarida Goulart   24. Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility             Tessy Joseph, Sabai Phyu, Su Yun Se-Thoe, and Justin Jang Hann Chu   25. Methods of SARS-CoV-2 Inactivation             Enyia R. Anderson, Tessa Prince, Lance Turtle, Grant L. Hughes, and Edward I. Patterson

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