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Overview"Actinomycetes are a group of Gram-positive bacteria of which many representatives are prominent for being prolific producers of bioactive natural products including antibiotics, fungicides, antitumor agents, or immunosuppressants. SARP transcriptional regulators are widely distributed among actinomycetes, especially in streptomycetes and are known to activate antibiotic biosynthesis. The set of genes responsible for the production of natural products, including pathway specific transcriptional regulators such as SARPs, are typically located in contiguous regions of the genome known as ""biosynthetic gene clusters"" (BGCs). In this book, Oona Rössler reports on the activation of antibiotic BGCs in selected actinomycetes strains upon heterologous expression of the SARP-type regulator PapR2 from Streptomyces pristinaespiralis. Applying a bioinformatic screening for the abundance of SARP genes and SARP consensus sequences as part of BGCs, the author has selected actinomycetes candidate strains from the DSMZ strain collection for heterologous SARP expression. It is shown that overexpression of papR2 increased the production of predominantly unknown antimicrobial compounds in more than half of the selected actinomycetes strains, as observed by bioassays against different microbial test strains including bacteria and fungi. " Full Product DetailsAuthor: Oona RösslerPublisher: Springer Fachmedien Wiesbaden Imprint: Springer Spektrum ISBN: 9783658445515ISBN 10: 3658445513 Pages: 104 Publication Date: 24 June 2024 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Forthcoming Availability: Not yet available This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release. Table of ContentsIntroduction.- Material and Methods.- Results.- Discussion.- References.- Acknowledgments.ReviewsAuthor InformationOona Rössler performed her Master Thesis at the Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures. Tab Content 6Author Website:Countries AvailableAll regions |