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Author:   Keith Redenbaugh ,  William R. Hiatt (The Colorado Prevention Center, Denver, Colorado, USA) ,  Belinda Martineau ,  Matthew Kramer
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Weight:   0.975kg
ISBN:  

9780849348037

ISBN 10:   084934803
Pages:   288
Publication Date:   22 May 1992
Audience:   Professional and scholarly ,  General/trade ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Overview. Request for Advisory Opinion. FOOD SAFETY. IDENTITY OF FLAVR SAVRÔ TOMATO. Common or Usual Name. Formal Name. Synonyms and Tradenames. Chemical Formulae and Molecular Weights. Composition of Mixtures. Purity and Specifications. Methods of Analysis of FLAVR SAVR™ Tomatoes. Description of Manufacturing Process. Appendix A-1: Variation in the Physical, Nutritional, and Chemical Composition of Tomatoes. Appendix A-2: University of California, Davis, Report on Variation in Constituents of Tomato. Appendix A-3: Methods of Genetic Modification and Their Use. Appendix A-4: Molecular Characterization of Tomato Fruit Polygalacturonase. Appendix A-5: Reduction of Polygalacturonase Activity in Tomato Fruit by Antisense RNA. Appendix A-6: Agrobacterium as a Gene Transfer Agent for Plants. Appendix A-7: Improved Binary Vectors for Agrobacterium-Mediated Plant Transformation. Appendix A-8: Binary Vectors, pCGN1416 and pCGN1436, Containing the FLAVR SAVR™ Gene. Appendix A-9: Nucleotide Sequence of the T-DNA Region of pCGN1436. Appendix A-10: Efficient Transfer of a Glyphosate Tolerance Gene into Tomato Using a Binary Agrobacterium tumefaciens Vector. Appendix A-11: Tomato Transformation and Regeneration with pCGN1436. Appendix A-12: Comparison of Agrobacterium-Mediated Transformation to Classical Breeding. Appendix A-13: Control of Fruit Ripening. USES OF FLAVR SAVRÔ Tomato. Introduction to Tomato. Current Uses-Processing and Fresh Market Tomatoes. Proposed Use-FLAVR SAVR™ Tomatoes. Use Levels. Fate in Foods. Human Estimated Daily Intake (EDI) of FLAVR SAVR™ Tomato. Background Exposure to Components of the FLAVR SAVR™ Tomato. Labeling. Appendix B-1: Fresh Market Tomatoes. Agricultural Statistics 1990. Appendix B-2: Processing Tomatoes. Production Statistics. Appendix B-3: A Complete Course in Canning. Appendix B-4 Commercial Processing of Tomatoes. INTENDED TECHNICAL EFFECT OF FLAVR SAVRÔ TOMATOES. Intended Technical Effect. Demonstration of Intended Technical Effect. Minimum Amount to Accomplish Intended Technical Effect. Appendix C-1: The Application of Antisense RNA Technology to Plants. Appendix C-2: Product Selection. Appendix C-3: Field Evaluation of Tomatoes with Reduced Polygalacturonase by Antisense RNA. Appendix C-4: Effects of Reduced PG on Tomato Fruit. Appendix C-5: Processing Data. Appendix C-6: Biochemical Analysis of Processed Samples. Appendix C-7: Sensory Evaluation of Transformed Tomatoes Containing the FLAVR SAVR™ Gene. ANALYTICAL METHODOLOGY AND RESULTS. Relevant Analyses. Methods Needed to Detect and Determine Concentrations. Sample Preparation and Analysis. Analytical Results. Discussion of Methods and Analytical Results. Appendix D-1: Selection of Eight Lines of FLAVR SAVR™ Tomatoes for Analysis. Appendix D-2: Characterization of the Inserted FLAVR SAVR™ and kanr Genes by Southern Blot Analyses. Appendix D-3: Kanamycin Germination Assay. Appendix D-4: PG Extraction and Assay of Tomato Fruit. Appendix D-5: Tomato Processing Analysis. Materials and Methods. Appendix D-6: National Food Laboratory Methods for Compositional Analysis. Appendix D-7: Results of Compositional Analysis. Appendix D-8: Tomatine Data. Appendix D-9: DNA Isolation Procedure. SAFETY INVESTIGATIONS ON FLAVR SAVRÔ TOMATOES. Introduction. Identifying the Concerns. Analytical Results. Discussion of Methods and Analytical Results. Safety Evaluation. Appendix E-1: Naturally Occurring Toxins. Appendix E-2: Material Safety Data Sheets for Tomatine and Tomatidine. Appendix E-3: Genetics of Lycopersicon. Appendix E-4: Acute Oral Toxicity Test (Limit Test) of Tomatoes in Rats. LIST OF PREPARERS. LIST OF REFERENCES. INDEX.

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