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This dissertation, Role of IRF4 in Regulating B Cells Development in Mice by Lan-yan, Lilian, Lam, 林蘭欣, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Interferon regulatory factor (IRF) family is a broadly expressed group of transcription factors that identified can be stimulated by type I interferon (IFN), among which IRF4, one of the members of IRF family, is widely expressed in immune system. In this project, using IRF-4 deficient (KO) mice, I have characterized B cell development and maturation in the immune system of these mice. To study which stages that B cell development was impaired, both cellularity and phenotypic analysis of B cell subpopulations in various lymphoid organs and body fluid, i.e. bone marrow, peritoneal cavity, blood, spleen, mediastinal lymph node and thymus from IRF-4KO mice and control mice were examined flow cytometry. To examine B cell development at various stages, B220+CD43+IgM- was used for progenitor B cells, B220+CD43-IgM- for precursor B cells and B220+CD43-IgM+ for immature B cells; In peritoneal cavity: B220+CD11b+ for B1 cells, B220+CD11b- for B2 cells, B220+CD11b+CD5+ for B1a cells and B220+CD11b+CD5- for B1b cells; for blood: CD19+ for B cells; CD4+ for CD4-T cells and CD8+ for CD8-T cells; for spleen and lymph node: IgMhi/IgDlo/CD19+ for follical mantle cells, IgMlo/IgDhi/CD19+ for T1 cells, IgMhi/IgDhi/CD19+ for T2 cells, CD21hiCD23-CD19+ for marginal zone B cells, CD21modCD23+CD19+ for follicular B cells, CD138+CD19- for plasma cell, CD138+CD19+ for plasma-blast cell, CD19+CD27+ for memory B cell and CD19+GL7+CD95+ for germinal center B cell, CD19+CD4+ for CD4-T cell and CD19+CD8+ for CD8-T cell; for thymus: CD4+CD3+ for T helper cell, CD8+CD3+ for T cytotoxic cell and CD4+CD8+CD3+ for immature T cell. In IRF-4KO mice, IgM + immature B cells in the bone marrow and CD19+ CD138+ plasma cells in the spleen were significantly reduced. Notably, CD11b+CD5+ B1 cells in the peritoneal cavity were also reduced. However, T cell development in the thymus was not affected. Together, these results have demonstrated that IRF-4 plays a role during B cell developmental process in bone marrow and plasma cell formation in the peripheral lymphoid organs while IRF-4 is not required for T cell development. Subjects: B cellsInterferon

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