Overview

The maturation of the precursors of mRNA to a functional transcript in eukaryotic cells is a complex, multistep process. It includes the formation of RNP complexes and the splicing procedure. Also, the presence of snRNA packaged as snRNP particles plays an important role in this process. In addition, snRNPs are involved in various autoimmune diseases in which autoantibodies are directed against components of these particles. Relevant techniques which are used to investigate RNA processing, splicing, protein-RNA complex formation and interactions as well as autoantibodies in basic and clinical research are presented in this laboratory manual. The book features easy-to-follow, step-by-step protocols and provides background information and full practical details.

Full Product Details

Author:   Johannes Schenkel
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   1998 ed.
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.400kg
ISBN:  

9783540624486

ISBN 10:   3540624481
Pages:   225
Publication Date:   23 January 1998
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Spiral bound
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Frank Jung, Constantin E. Sekeris, and Johannes Schenkel: Isolation and Immunochemical Characterization of hnRNP Particles.-Ruth Sperling and Joseph Sperling: The lnRNP Particle -- A Naturally Assembled Complex of Pre-mRNA and Splicing Factors.-Karl Otto Greulich: Intrinsic Fluorescence Techniques for Studies on Protein-Protein and Protein-RNA Interactions in RNP Particles.-Ulf Skoglund, Lars-Göran Öfverstedt, and Bertil Daneholt: Procedures for Three-Dimensional Reconstruction from Thin Sections with Electron Tomography.-Berthold Kastner: Purification and Electron Microscopy of Spliceosomal snRNPs.-Panayiotis G. Vlachoyiannopoulos: Detection of Autoantibodies to Extractable Cellular Antigens.-Martin Blüthner: Methods in Immunolocalization of Autoantigens.- Johannes Schenkel, Frank Jung, Apostolia Guialis, and Angela Krämer: In Vitro Splicing of Pre-mRNA in HeLa Extracts.

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