Overview

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

Full Product Details

Author:   Frank J. Schmidt
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   1240
Dimensions:   Width: 17.80cm , Height: 1.20cm , Length: 25.40cm
Weight:   4.438kg
ISBN:  

9781493941612

ISBN 10:   1493941615
Pages:   219
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique.- RNA-directed Recombination of RNA in Vitro.- RNA-RNA Selex.- Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System.- Trans-activation of Large Ribozymes.- Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies.- Single Molecule Studies of RNA-RNA Interactions.- Modification Interference Analysis of the Ribosome.- Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping.- Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA.- Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.- Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by Small Angle X-ray Scattering.- Fusion RNAs in Crystallographic Studies of Double-stranded RNA from Trypanosome RNA Editing.

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