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Full Product Details

Author:   Christopher W.J. Smith (Lecturer in Biochemistry, University of Cambridge)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   192
Dimensions:   Width: 15.70cm , Height: 2.00cm , Length: 23.50cm
Weight:   0.625kg
ISBN:  

9780199636501

ISBN 10:   0199636508
Pages:   366
Publication Date:   09 July 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1: Applications of chemically synthesized RNA 2: Preparation of RNA-Protein complexes for X-ray crystallography and NMR 3: Uses of site-specifically modified RNAs constructed by RNA ligation 4: The Electrophoretic Mobility Shift Assay for RNA Binding Proteins 5: Affinity methods for isolating RNA-binding proteins 6: Synthetic lethal/enhancer screening to identify snRNA-protein and protein-protein interactions in yeast pre-mRNA splicing 7: Detecting RNA-Protein Interactions and Isolating cDNA Clones by Northwestern Screening 8: A three-hybrid system to detect and analyse RNA-protein interactions in vivo 9: In vivo selection of specific RNA-binding polypeptides using a transcription anti-termination reporter assay 10: Foot-printing and modification-interference analysis of binding sites on RNA 11: Analysis of RNA-Protein and RNA-RNA contacts between ribosomes and functional ligands using site-directed cross-linking techniques 12: In vitro selection of nucleic acid ligands

Reviews

<br> There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --The Quarterly Review of Biology<br>

There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover.--The Quarterly Review of Biology

There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --TheQuarterly Review of Biology

There are abundant opportunities for RNA-protein interactions in the cell. Many different levels of activity need the function of RNA and proteins complexes . . . Readers will find here a variety of techniques for the preparation of specific RNA samples by chemical synthesis . . ., in vitro transcription . . . or a combination of both through RNA ligation . . . This book will be accessible to readers unfamiliar with the field. The twelve chapters contain a good presentation of each approach by discussing applications, potentials, limits and prospectives. Step-by-step experimental protocols are given as they were used in specific projects, but great care has been taken to provide detailed discussion so that they may be modified for use by readers in their own research. This volume is a remarkable resource that, no doubt, still offers many mysteries for us to uncover. --The Quarterly Review of Biology<br>

Author Information

Dr Christopher W J Smith, Lecturer in Biochemistry, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Old Addenbrooke's Site, Cambridge CB2 1GA. Tel: 01223 333655 / 333665. Fax: 01223 766002. Email: cwjs1@mole.bio.cam.ac.uk

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