RNA Methylation: Methods and Protocols

Author:   Alexandra Lusser
Publisher:   Humana Press Inc.
Edition:   1st ed. 2017
Volume:   1562
ISBN:  

9781493968053


Pages:   304
Publication Date:   28 March 2017
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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RNA Methylation: Methods and Protocols


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Author:   Alexandra Lusser
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2017
Volume:   1562
Dimensions:   Width: 17.80cm , Height: 1.90cm , Length: 25.40cm
Weight:   7.392kg
ISBN:  

9781493968053


ISBN 10:   149396805
Pages:   304
Publication Date:   28 March 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

LC-MS Analysis of Methylated RNA.- Comparative Analysis of Ribonucleic Acid Digests (CARD) by mass Spectrometry.- Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation.- Genome-Wide Location Analyses of N6-Methyladenosine Modifications (m6A-seq).- Mapping m6A at Individual-Nucleotide Resolution using Crosslinking and Immunoprecipitation (miCLIP).- Detection and Quantification of N6-Methyladenosine in Messenger RNA by TLC.- Illustrating the Epitranscriptome at Nucleotide Resolution using Methylation-iCLIP (miCLIP).- Detection of 5-Methylcytosine in Specific Poly(A) RNAs by Bisulfite Sequencing.- Transcriptome-Wide Detection of 5-Methylcytosine by Bisulfite Sequencing.- Analysis of High-Throughput RNA Bisulfite Sequencing Data.- Statistcal Methods for Transcriptome-Wide Analysis of RNA Methylation by Bisulfite.- High-Throughput Mapping of 2’-O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol).- RiboMeth-seq: Profiling of 2’-O-Me in RNA.- In Silico Identification of RNA Modifications from High-Throughput Sequencing Data using HAMR.- High-Throughput Small RNA Sequencing Enhanced by AlkB-Facilitated RNA de-Methylation (ARM-seq).- Transcriptome-Wide Mapping of N1-Methyladenosine Methylome.- In Vitro Assays for RNA Methyltransferase Activity.- Crosslinking Methods to Identify RNA Methyltransferase Targets In Vivo.- Methylated mRNA Nucleotides as Regulators for Ribosomal Translation.- Automated Chemical Solid-Phase Synthesis and Deprotection of 5-Hydroxymethylcytosine-Containing RNA.

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