Overview

This collection explores the latest advances in riboregulators, such as RNA-only systems and ribonucleoprotein systems, and provides detailed techniques to study, evolve, and design them. Beginning with a set of chapters focused on the design and application of small RNA (sRNA) regulator systems, the book continues with sections on techniques to create switchable riboregulator systems known as riboswitches, technologies that leverage RNA-guided CRISPR-Cas systems to edit the epigenome, control gene expression, and create diagnostics, as well as computational and experimental techniques to investigate the sequence-structure-function relationship of RNA systems that can both advance fundamental understanding and rational design of riboregulators. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Riboregulator Design and Analysis is an ideal guide for scientists and engineers interested in the design and application of riboregulators and driving further innovation in the field.

Full Product Details

Author:   James Chappell ,  Melissa K. Takahashi
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2518
Weight:   0.662kg
ISBN:  

9781071624234

ISBN 10:   1071624237
Pages:   333
Publication Date:   08 June 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

RNP-Based Control Systems for Genetic Circuits in Synthetic Biology Beyond CRISPR.- Computational Design of RNA Toehold-Mediated Translation Activators.- Design of RNA-Based Translational Repressors.- Design of Ribocomputing Devices for Complex Cellular Logic.- Computational Design of Small Transcription Activating RNAs (STARs).- Design and Assembly of Multi-Level Transcriptional and Translational Regulators for Stringent Control of Gene Expression.- Model-Based Design of Synthetic Antisense RNA for Predictable Gene Repression.- Design of a Toolbox of RNA Thermometers.- Development of Synthetic Riboswitches to Guide the Evolution of Metabolite Production in Microorganisms.- Efficient Method to Identify Synthetic Riboswitches Using RNA-Based Capture-SELEX Combined with In Vivo Screening.- RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing.- Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular CRISPRa System.- Reprogramming TracrRNAs for In Vitro RNA Detection and In Vivo Transcriptional Recording.- Harnessing CRISPR-Cas9 for Epigenetic Engineering.- RNA Structure Prediction, Analysis, and Design: An Introduction to Web-Based Tools.- Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions.- Cotranscriptional RNA Chemical Probing.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions