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Author:   Francisco Rivero
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2012
Volume:   827
Weight:   1.087kg
ISBN:  

9781493958962

ISBN 10:   1493958968
Pages:   407
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Historical Overview of Rho GTPases.- Rho GTPases: Deciphering the Evolutionary History of a Complex Protein Family.- Biochemical Assays to Characterize Rho GTPases.- How to Analyze Bacterial Toxins Targeting Rho GTPases.- Assessing Ubiquitylation of Rho GTPases in Mammalian Cells.- Posttranslational Lipid Modification of Rho Family Small GTPases.- Analysis of the Role of RhoGDI1 and Isoprenylation in the Degradation of Rho GTPases.- A Quantitative Fluorometric Approach for Measuring the Interaction of RhoGDI with Membranes and Rho GTPases.- Rho GTPases and Cancer Cell Transendothelial Migration.- Quantitative and Robust Assay to Measure Cell-cell Contact Assembly and Maintenance.- Rho GTPase Knockout Induction in Primary Keratinocytes from Adult Mice.- Rho GTPase Techniques in Osteoclastogenesis.- Assessment of Rho GTPase Signaling During Neurite Outgrowth.- Assesment of the Role for Rho Family GTPases in NADPH Oxidase Activation.- Multiplex Imaging of Rho GTPase Activities in Living Cells.- FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor Mediated Phagocytosis in Macrophages.- High Throughput Flow Cytometry Bead-Based Multiplex Assay for Identification of Rho GTPase Inhibitors.- Functional Analysis of RhoGTPase Activation and Inhibition in a Bead-Based Miniaturized Format.- Use of Phage Display For the Identification of Molecular Sensors Specific for Activated Rho.- Identification of New Interacting Partners for Atypical Rho-GTPases: a SILAC-Based Approach.- Using Zebrafish for Studying Rho GTPases Signaling in Vivo.- Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans.- Protocol for ex vivo Incubation of Drosophila Primary Post-Embryonic Haemocytes for Real-Time Analyses.- Analysis of Rho GTPase Activation in Saccharomyces cerevisiae.- Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum.- FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes.- Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum.- FRAP-Based Analysis of Rho GTPase-dependent Polar Exocytosis in Pollen Tubes.

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