Rhinoviruses: Methods and Protocols

Author:   David A. Jans ,  Reena Ghildyal
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   1221
ISBN:  

9781493955336


Pages:   190
Publication Date:   23 August 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Rhinoviruses: Methods and Protocols


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Author:   David A. Jans ,  Reena Ghildyal
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   1221
Dimensions:   Width: 17.80cm , Height: 1.10cm , Length: 25.40cm
Weight:   3.931kg
ISBN:  

9781493955336


ISBN 10:   1493955330
Pages:   190
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Classification and Evolution of Human Rhinoviruses.- Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples.- Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples.- Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing.- Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions.- Propagation of Rhinovirus – C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface.- Infectivity Assays of Human Rhinovirus-A and –B Serotypes.- Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating.- Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research.- Proteases of Human Rhinovirus – Role in Infection.- A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays.- Reverse Genetics System for Studying Human Rhinovirus Infections.- Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag.- Mouse Models of Rhinovirus Infection and Airways Disease.

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