Overview

  This volume details the history of Retinitis Pigmentosa and current treatment options. Chapters guide readers through CRISPR, gene therapy, stem cell therapy, next-generation sequencing methods, gene editing, and translational applications of other therapies to the treatment of Retinitis Pigmentosa. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Retinitis Pigmentosa aims to be a useful practical guide to researches to help further their study in this field.   

Full Product Details

Author:   Stephen H. Tsang ,  Peter M.J. Quinn
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2560
Weight:   0.990kg
ISBN:  

9781071626504

ISBN 10:   1071626507
Pages:   404
Publication Date:   09 December 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Introduction and History of Retinitis Pigmentosa Hung-Hsi Wang, Nelson Chen, Nan-Kai Wang   2 Etiology of Retinitis Pigmentosa Mark P. Breazzano, Maeher Grewal, Stephen H. Tsang, and Royce W.S. Chen   3. Clinical Evaluation of Patients with Retinitis Pigmentosa Sitara H. Hirji   4. Molecular Genetic Testing Approaches for Retinitis Pigmentosa Megan Soucy, Akemi Joy Tanaka, and Avinash Dharmadhikari   5. Pedigree Analysis of Families and Patients Affected by Retinitis Pigmentosa Ioana Scherbakova and Sara D. Ragi   6. Whole Genome Sequencing for Detection of Structural Variants in Patients with Retinitis Pigmentosa Alexander H. Chai   7. Fundus Imaging Methodologies to Assess RP patients Jin Kyun Oh, Joseph Ryu, and Stephen H Tsang   8. The Use of Optical Coherence Tomography in Evaluation of Retinitis Pigmentosa Ha Min Kim, Jin Kyun Oh, Stephen H. Tsang   9. Optical Coherence Tomography Angiography (OCTA) findings in Retinitis Pigmentosa Vlad Diaconita and Wei Kiong Ngo   10. ERG to Evaluate the Retina in Cases of Retinitis Pigmentosa Wan-Chun Huang, Pei-Kang Liu, and Nan-Kai Wang   11. Visual Function Tests: Visual Fields Angela Y. Chang   12. Visual Function Tests Ritah Chumdermpadetsuk, Sara D. Ragi, and Stephen H. Tsang   13. Visual Acuity as a Measurement of Visual Function Zachary E. Snow   14. Measure of Visual Function Sitara H. Hirji   15. Ophthalmic Fluorescein Angiography Divin R. Baddam, Sara D. Ragi, Stephen H. Tsang, and Wei Kiong Ngo   16. Indocyanine Green Angiography Divin R. Baddam, Sara D. Ragi, Stephen H. Tsang, and Wei Kiong Ngo   17. Use of the Medmont Dark-Adapted Chromatic Perimeter for Assessing Rod Function in Retinitis Pigmentosa Yan Nuzbrokh, Sara Ragi, and Stephen H. Tsang   18. Multi-Luminance Mobility Testing Endpoint Henry W. Zhou   19. Preclinical Models of Retinitis Pigmentosa Mark D. Travor   20. ERG to evaluate the retina using mouse models Pei-Kang Liu, Wan-Chun Huang, and Nan-Kai Wang   21. Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent Chia-Hua Cheng and Xuan Cui   22. Large animal models of retinitis pigmentosa in therapy development and preclinical testing Laurence M Occelli and Simon M Petersen-Jones   23. CRISPR manipulations in stem cell lines Ya-Ju Chang, Xuan Cui, Sarah R. Levi, Laura A. Jenny, and Stephen H. Tsang   24. Metabolite extraction from RPE cells and retinas related to retinitis pigmentosa Xuan Cui, Ya-Ju Chang, Laura A. Jenny, Sarah R. Levi, Jianhai Du, and Stephen H. Tsang   25. CRISPR-mediated genome engineering in cell lines Ya-Ju Chang, Joseph Ryu, Xuan Cui, and Stephen H Tsang   26. CRISPR off-targeting Christine L. Xu, Merry Zhechao Ruan, Sara D. Ragi, and Stephen H. Tsang   27. CRISPR manipulations in stem cell lines Ya-Ju Chang, Xuan Cui, Sara D. Ragi, and Stephen H. Tsang   28. Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene Delivery Amy Tso, Bruna Lopes da Costa, Laura A. Jenny, Sarah R. Levi, Alexandra Fehnel, Sara D. Ragi, Yao Li, and Peter M.J. Quinn   29. Culture of Human Retinal Explants for Ex Vivo Assessment of AAV Gene Delivery Wen-Hsuan Wu, Amy Tso, Mark P. Breazzano, Sarah R. Levi, Laura A. Jenny, Stephen H. Tsang, and Peter M.J. Quinn   30. Prime Editing for the Installation and Correction of Mutations causing Inherited Retinal Disease: A Brief Methodology Yi-Ting Tsai, Bruna Lopes da Costa, Nicholas D. Nolan, Salvatore M. Caruso, Laura A. Jenny, Sarah R. Levi, Stephen H. Tsang, and Peter M.J. Quinn   31. Large scale single-nucleus RNA sequencing compatible with complex archived samples Chao-Yu Liu and Hsu-Hsin Chen   32. Vector Shedding and Immunogenicity Sampling in AAV-based Gene Therapy for Retinitis Pigmentosa Patients Ahra Cho   33. Current Management Options for Patients with Retinitis Pigmentosa Abinaya Thenappan   34. Challenges of Treatment Methodologies and the Future of Gene Therapy and Stem Cell Therapy to Treat Retinitis Pigmentosa Angela Y. Chang   35. Ocular Injection Techniques for Retinitis Pigmentosa: Intravitreal, Subretinal, and Suprachoroidal Ioana Scherbakova, Sara D. Ragi, and Tarun Sharma   36. Surgical Approach with Pars Plana Vitrectomy for Subretinal Gene Therapy Mark P. Breazzano, Sean A. Batson, Stephen H. Tsang, and Royce W.S. Chen

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