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Author:   Livia S. Carvalho
Publisher:   Springer-Verlag New York Inc.
Imprint:   Humana
ISBN:  

9781071651971

ISBN 10:   1071651978
Pages:   238
Publication Date:   17 April 2026
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Forthcoming
Availability:   Not yet available  
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Table of Contents

Selecting Novel Retino-Tropic AAV Capsids by Directed Evolution.- Evaluation of CRISPR/CasRx-Mediated VEGF mRNA Knockdown in Mouse Retina.- Optimized Retinal Cell Dissociation for Downstream Analysis of Cone Photoreceptors.- Development of In Vitro Potency Assays for Gene Therapy Products Targeting cGMP-Related Retinopathies.- Quantification of Drusen-Like Deposits in Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cells Using Confocal Microscopy.- Ex Vivo Culture and Formalin-Fixed Paraffin Embedding of Murine Retinal Explants.- Culture of Human Retinal Explants as an Ex Vivo Model for Retinal Gene Therapy.- Electrophysiological Characterization of Photoreceptor-Like Cells in Retinal Organoids Using Whole-Cell Patch Clamp Method.- Generation and Characterization of Patient-Derived Human Pluripotent Stem Cell Lines for Retinal Gene Therapy.- Transduction of AAV Vectors in iPSC-Derived Retinal Organoids.- Intravitreal Injections in Mouse Models of Eye Disease.- Behavioral Assessment of Vision in Dogs Using a Four-Alternative Choice-Based Testing Device.- Behavioral Vision Testing Using an Automated Observational Tracking System (VisioBox) to Obtain a Measure of Visual Acuity in Larval Zebrafish.- Design and Validation of Mirtron-Mediated RNA Interference for Gene Therapy.- Applications of Fundus-Tracked Perimetry in the Assessment of Visual Sensitivity.- Assessing Visual Function in Retinal Gene Therapy.

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