Overview

Research Protocols for Ophthalmic Disease Mechanisms and Therapeutics: Glaucoma - Ocular Hypertension offers a detailed, step-by-step protocols for isolating, characterizing, and analyzing cells and tissues of the eye's anterior segment-where OHT originates-using techniques from molecular biology, pharmacology, and histology. Across eight thematic sections, the book covers ocular anatomy, drug screening, receptor localization, cell-based assays, in vitro and in vivo models, drug formulation, and cutting-edge technologies such as gene editing, single-cell RNA sequencing, and stem cell therapies. Each protocol is designed to support reproducibility and translational relevance. Key Features: - Details validated protocols for drug discovery and disease modeling. - Demonstrates methods for studying receptor function and pharmacokinetics. - Provides animal models and ex vivo systems to evaluate therapeutic strategies. - Incorporates emerging tools such as CRISPR, metabolomics, and exosomes. - Bridges experimental design with clinical relevance for glaucoma research.

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