Overview

This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and  bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Purnima Dubey
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1790
Weight:   4.391kg
ISBN:  

9781493978588

ISBN 10:   1493978586
Pages:   221
Publication Date:   02 June 2018
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Dual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function.- Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging.- Optical Bioluminescence Protocol for Imaging Mice.- Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase.- Reporter Based BRET Sensors for Measuring Biological Functions In Vivo.- Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains.- In vivo Bacterial Imaging using Bioluminescence.- Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging.- Hypoxia-induced Reporter Genes with Different Half-lives.- Visualization of Immune System Reconstitution by Bioluminescent Imaging.- Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo.- Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes.- Imaging T Cell Dynamics and Function byPET using Human Nuclear Reporter Genes.- Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT.- Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes.- Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models.

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