Overview

Leading experts distill their extensive experience into user-friendly methods for the study of fluid-electrolyte homeostasis and kidney function in health and disease. Described in step-by-step detail, the techniques move from the molecular level to the whole organism, from simple models to integrative physiology. Here the researcher will find established disease models, methods for optimizing renal disease research, as well as much help in choosing imaging techniques for studies of the kidney's structure and function in health, disease, and during embryonic development. Subjects covered include molecular diagnostics, strategies for studying the molecular mechanisms of kidney disease, technical means to assess the functional correlates of disease, and the planning of clinical trials.

Full Product Details

Author:   Michael S. Goligorsky
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. Softcover of orig. ed. 2003
Volume:   86
Dimensions:   Width: 15.20cm , Height: 2.90cm , Length: 22.90cm
Weight:   0.836kg
ISBN:  

9781617373800

ISBN 10:   161737380
Pages:   500
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Optimizing The Usage of Models Renal Disease.- Standards of Animal Care in Biological Experiments.- Models of Polycystic Kidney Disease.- Rat Models of the Metabolic Syndrome.- Models of Glomerulonephritis.- Choices of Imaging Techniques in Studies of Renal Disease.- Functional Studies of the Kidney with Magnetic Resonance Imaging.- Use of Radionuclides to Study Renal Function.- Intravital Videomicroscopy.- Confocal and Two-Photon Microscopy.- Atomic Force Microscopy in Renal Physiology.- Freeze-Fracture Analysis of Renal-Epithelial Tight Junctions.- Studies of Renal Development.- Organ Culture of Intact Metanephric Kidneys.- The Ureteric Bud.- Studies of Cell Lineage in the Developing Kidney.- Transient Transfection Assays for Analysis of Signal Transduction in Renal Cells.- Approaches to Study Molecular Mechanisms of Disease.- The Study of Gene Polymorphisms.- Laser-Capture Microdissection.- Serial Analysis of Gene Expression.- RNA Labeling and Hybridization of DNA Microarrays.- Gene-Expression Analysis of Microdissected Renal Biopsies.- The Use of SELDI ProteinChip® Array Technology in Renal Disease Research.- Technical Means to Assess Functional Correlates of Disease.- Clearance Studies in Genetically Altered Mice.- Long-Term Blood-Pressure Monitoring in Unrestrained Animals.- In Vitro Studies on Renin Release.- Mitochondrial Function.- Detection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney Proteins.- Products of Arachidonic Acid Metabolism.- Methods for Measurements of Heme Oxygenase (HO) Isoforms-Mediated Synthesis of Carbon Monoxide and HO-1 and HO-2 Proteins.- The Juxtamedullary Nephron Preparation.- Tubuloglomerular Feedback.- Microdissected Perfused Vessels.- Renal Microperfusion Techniques.- How to Design a Clinical Trial.- Mapping theDisease.

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