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Author:   George Kassiotis ,  Adrian Liston
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   707
Weight:   0.566kg
ISBN:  

9781493961580

ISBN 10:   1493961586
Pages:   282
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Regulatory T Cells: History and Perspective.- In vitro Treg Suppression Assays.- Generation of T Cell Hybridomas from Naturally-Occurring FoxP3+ Regulatory T Cells.- In vitro and In vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells.- Flow Cytometric Profiling of Mature and Developing Regulatory T Cells in the Thymus.- ChIP-on-Chip for FoxP3.- Live Imaging of Dendritic Cell-Treg Cell Interactions.- Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In vivo.- In vivo Treg Suppression Assays.- In vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model.- Antigen-Specific Induction of Regulatory T Cells In vivo and In vitro.- In vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection.- Analysis of Human FoxP3+ Treg Cells Phenotype and Function.- Depletion of Human Regulatory T Cells.- Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In vitro Assay.- Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose.- Flow Cytometric Detection of Human Regulatory T Cells.

Reviews

From the reviews: This book is to provide a detailed and up-to-date collection of methods and protocols utilised for the analysis of regulatory T cells. ... this book would be of most use to scientific researchers, in particular PhD students and post-doctoral researchers, working in the field of regulatory T cells within a laboratory setting. For individuals using in vitro or in vivo mouse models for regulatory T cell analysis, this book would be a perfect asset. (Karen Smith, Immunology News, February, 2012) This volume in the Methods in Molecular Biology series is a collection of current techniques to study regulatory T cells. ... Researchers who need to discover techniques to isolate or use regulatory T cells are the primary audience. The authors of the individual chapters are knowledgeable about the techniques they describe. ... There are other books that discuss regulatory T cells, but not with the level of procedural detail that this one has. It would be a useful reference for a library ... . (Marion C. Cohen, Doody's Book Reviews, August, 2011)

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