Overview

Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport. This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.

Full Product Details

Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  W. E. Balch (The Scripps Research Institute, La Jolla, CA, USA) ,  Channing J. Der (Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   v. 329
Weight:   0.910kg
ISBN:  

9780121822309

ISBN 10:   0121822303
Pages:   553
Publication Date:   08 February 2001
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Unknown
Availability:   Out of stock  

Table of Contents

Section I. Rab GTPases 1: Structural Basis for Rab Function: An Overview 2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis 3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase 4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases 5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms 6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family 7: Purification and Properties of Rab3 GDP/GTP Exchange Protein 8: Purification and Properties of Rab3 GTPase-Activating Protein 9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins 10: Doc2α as Modulator of Ca2+-Dependent Exocytosis 11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases 12: Purification and Characterization of Yeast Exocyst Complex 13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling 14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays 15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-5 16: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis 17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A 18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network 19: Expression and Properties of Rab7 in Endosome Function 20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport 21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit 22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters 23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells 24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian Counterpart Section II. ADP-Ribosylation Factor (ARF) GTPases 25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins 26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways 27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements 28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1) 29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6) 30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate 31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast 32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor 33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-1 34: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo3 35: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain 36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins 37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins 38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF) 39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic 40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes 41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes 42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex 43: Purification, Properties, and Analysis of yARL3 44: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1) Section III. Sar GTPases 45: Purification and Properties of Rat Liver Sec23–Sec24 Complex 46: Purification of Functional Sec13p–Sec31p Complex, A Subunit of COPII Coat Section IV. Dynamin GTPases 47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-1 48: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin 49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments 50: Interactions of Dynamin and Amphiphysin with Liposomes 51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Proteins Section V. Septin GTPases 52: Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis Author Index Subject Index

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