Overview

This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Full Product Details

Author:   Jacqueline MacDonald ,  Igor Kolotilin ,  Rima Menassa
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2016
Volume:   1385
Dimensions:   Width: 17.80cm , Height: 1.90cm , Length: 25.40cm
Weight:   7.318kg
ISBN:  

9781493932887

ISBN 10:   1493932888
Pages:   296
Publication Date:   27 November 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology.- Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors.- Virus-Derived Vectors for the Expression of Multiple Proteins in Plants.- Transient Protein Expression by Agroinfiltration In Lettuce.- Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii.- Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes.- Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation.- Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants.- Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector.- Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots.- Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants.- Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor.- Liquid-Liquid Phase Separation of Oil Bodies From Seeds.- Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract.- Molecular Analyses of Transgenic Plants.- Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response.- Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2.- A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops.- Generating Transgenic Plants Showing Antibody-Mediated Pathogen Resistance.    

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions