Overview

Expert international research workers offer the most up-to-date and comprehensive collection of techniques available for expressing one or more genes of interest. The authoritative contributors describe in depth the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host. When combined with a companion volume by the same editor, Recombinant Proteins: Detection and Isolation Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.

Full Product Details

Author:   Rocky S. Tuan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   62
Dimensions:   Width: 15.50cm , Height: 3.30cm , Length: 23.50cm
Weight:   1.074kg
ISBN:  

9780896034808

ISBN 10:   0896034801
Pages:   521
Publication Date:   06 March 1997
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Recombinant Gene Expression Protocols.- Expression in E. Coli.- Designing Expression Plasmid Vectors in E. coli.- Design of Bacterial Hosts for lac-Based Expression Vectors.- Bacteriophage ?-Based Expression Vectors.- Strategies for Gene Fusions.- Application of the E. coli trp Promoter.- Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7.- Expression and Secretion of Proteins in E. coli.- Synthetic Two-Cistron Expression System.- Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation.- Expression in Yeast.- Yeast Plasmids.- Inducible Expression Cassettes in Yeast: GAL4.- Inducible Expression Cassettes in Yeast: ADH2.- Constitutive Expression Vectors: PGK.- Viral Expression Systems.- Design and Construction of Recombinant Vaccinia Viruses.- A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus.- Design of Retroviral Expression Vectors.- Use of Defective Herpes-Derived Plasmid Vectors.- Expression in Nonmammalian Higher Eukaryotes.- Use of Baculovirus Expression Vectors.- Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos.- Analysis of Heterologous Gene Expression in Xenopus Blastomeres.- Expression in Mammalian Cells.- Overview of Vector Design for Mammalian Gene Expression.- Experimental Strategies in Efficient Transfection of Mammalian Cells.- Experimental Strategies in Efficient Transfection of Mammalian Cells.- Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer.- Selection of Transfected Cells.- Selection of Transfected Cells and Coamplification of Transfected Genes.- Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells.- Expression in Transgenic Organisms.-Promoters to Express Cloned Genes Uniformly in Drosophila.- Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes.- Strategies in Generating Transgenic Mammals.- Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens.- Strategies for the Production of Transgenic Chickens.- Expression in Plants.- Plant Cell Transfection by Electroporation.- Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment.- Transformation of the Cereals Using Agrobacterium.- Transformation of Microalgae Using Silicon Carbide Whiskers.

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