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Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  Ray Wu (Division of Biological Sciences, Cornell University, Ithaca, New York, U.S.A.) ,  Lawrence Grossman (School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   154
Dimensions:   Width: 15.20cm , Height: 3.80cm , Length: 22.90cm
Weight:   0.980kg
ISBN:  

9780121820558

ISBN 10:   0121820556
Pages:   576
Publication Date:   11 December 1987
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Methods for Cloning cDNA. H. Okayama, M. Kawaichi, M. Brownstein, F. Lee, T. Yokota, and K. Arai, High-Efficiency Cloning of Full-Length cDNA; Construction and Screening of cDNA Expression Libraries for Mammalian Cells. G. Heidecker and J. Messing, A Method for Cloning Full-Length cDNA in Plasmid Vectors. D.C. Alexander, An Efficient Vector*b1Primer cDNA Cloning System. C. Coleclough, Use of Primer*b1Restriction End Adapters in cDNA Cloning. Identification of Cloned Genes and Mapping of Genes. A.A. Reyes and R.B. Wallace, Mapping of RNA Using S1 Nuclease and Synthetic Oligonucleotides. C.G. Miyada and R.B. Wallace, Oligonucleotide Hybridization Techniques. M. Snyder, D. Sweetser, R.A. Young, and R.W. Davis, *glgt 11: Gene Isolation with Antibody Probes and Other Applications. M.R. Gray, G.P. Mazzara, P. Reddy, M. Rosbash, Searching for Clones with Open Reading Frames. G.M. Weinstock, Use of Open Reading Frame Expression Vectors. J.D. Boeke, J. Trueheart, G. Natsoulis, and G.R. Fink, 5-Flouroorotic Acid as a Selective Agent in Yeast Molecular Genetics. F.J. de Bruijn, Transposon Tn5 Mutagenesis to Map Genes. J.D. Noti, M.N. Jagadish, and A.A. Szalay, Site-Directed Tn5 and Transplacement Mutagenesis: Methods to Identify Symbiotic Nitrogen Fixation Genes in Slow-Growing Rhizobium. Chemical Synthesis and Analysis of Oligodeoxynucleotides. R. Frank, A. Meyerhans, K. Schwellnus, and H. Blvcker, Simultaneous Synthesis and Biological Applications of DNA Fragments: An Efficient and Complete Methodology. H.W. DjurhuusYMatthes, A. Staub, and P. Chambon, The Segmented Paper Method: DNA Synthesis and Mutagenesis by Rapid Microscale ""Shotgun Gene Synthesis"". M.H. Caruthers, A.D. Barone, S.L. Beaucage, D.R. Dodds, E.F. Fisher, L.J. McBride, M. Matteucci, Z. Stabinsky, and J.-Y. Tang, Chemical Synthesis of Deoxyoligonucleotides by the Phosphoramidite Method. S.J. Horvath, J.R. Firca, T. Hunkapiller, M.W. Hunkapiller, and L. Hood, An Automated DNA Synthesizer Employing Deoxynucleoside 3*b7-Phosphoramidites. Site-Specific Mutagenesis and Protein Engineering. M.J. Zoller, Oliconucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template. W. Kramer and H.-J. Fritz, Oligonucleotide-Directed Construction of Mutations via Gapped Duplex DNA. T.A. Kunkel, J.D. Roberts, and R.A. Zakour, Rapid and Efficient Site-Specific Mutagenesis without Phenotypic Selection. P. Carter, Improved Oligonucleotide-Directed Mutagenesis Using M13 Vectors. D.F. Mark, A. Wang, and C. Levenson, Site-Specific Mutagenesis to Modify the Human Tumor Necrosis Factor Gene. R. Pine and P.C. Huang, An Improved Method to Obtain a Large Number of Mutants in a Defined Region of DNA. P. Kollman and W.F. van Gunsteren, Molecular Mechanics and Dynamics in Protein Design. S. Pongor, The Use of Structural Profiles and Parametric Sequence Comparison in the Rational Design of Polypeptides. J.W. Taylor and E.T. Kaiser, Structure*b1Function Analysis of Proteins through the Design, Synthesis, and Study of Peptide Models. C.R. Matthews, Effect of Point Mutations on the Folding of Globular Proteins. T. Alber and B.W. Matthews, Structure and Thermal Stability of Phage T4 Lysozyme. Author Index. Subject Index.

Reviews

Praise for the Volume This reviewer finds Methods in Enzymology Volume 154, Recombinant DNA Part E to be excellent reference book for persons working in molecular biology. --ADOLESCENT AND PEDIATRIC GYNECOLOGY Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

Praise for the Volume This reviewer finds Methods in Enzymology Volume 154, Recombinant DNA Part E to be excellent reference book for persons working in molecular biology. --ADOLESCENT AND PEDIATRIC GYNECOLOGY Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

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